Recentadvances in the development of peptide nucleic acid as a gene-targeted drug

被引:40
作者
Marin, VL [1 ]
Roy, S [1 ]
Armitage, BA [1 ]
机构
[1] Carnegie Mellon Univ, Dept Chem, Pittsburgh, PA 15213 USA
关键词
antigene; antisense; gene expression; hybridisation; peptide nucleic acid; PNA; telomerase; therapeutics;
D O I
10.1517/14712598.4.3.337
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Peptide nucleic acid (PNA) is a non-ionic mimic of DNA that binds to complementary DNA and RNA sequences with high affinity and selectivity. Targeting of single-stranded RNA leads,to antisense effects, whereas PNAs directed toward double-stranded DNA exhibit antigene properties. Recent advances in cell uptake and in antisense and antigene effects in biological systems are summarised in this review. in addition to traditional targets, namely genomic DNA and messenger RNA, applications for PNA as a bacteriocidal antibiotic, for regulating splice site selection and as a telomerase inhibitor are described.
引用
收藏
页码:337 / 348
页数:12
相关论文
共 107 条
[1]   Down-regulation of amyloid precursor protein by peptide nucleic acid oligomer in cultured rat primary neurons and astrocytes [J].
Adlerz, L ;
Soomets, U ;
Holmlund, L ;
Viirlaid, S ;
Langel, Ü ;
Iverfeldt, K .
NEUROSCIENCE LETTERS, 2003, 336 (01) :55-59
[2]   A peptide nucleic acid (PNA) is more rapidly internalized in cultured neurons when coupled to a retro-inverso delivery peptide.: The antisense activity depresses the target mRNA and protein in magnocellular oxytocin neurons [J].
Aldrian-Herrada, G ;
Desarménien, MG ;
Orcel, H ;
Boissin-Agasse, L ;
Méry, J ;
Brugidou, J ;
Rabié, A .
NUCLEIC ACIDS RESEARCH, 1998, 26 (21) :4910-4916
[3]   Functional and dysfunctional roles of quadruplex DNA in cells [J].
Arthanari, H ;
Bolton, PH .
CHEMISTRY & BIOLOGY, 2001, 8 (03) :221-230
[4]   Telomerase as a possible target for anticancer therapy [J].
Autexier, C .
CHEMISTRY & BIOLOGY, 1999, 6 (11) :R299-R303
[5]   Synthesis and characterization of a peptide nucleic acid conjugated to a D-peptide analog of insulin-like growth factor 1 for increased cellular uptake [J].
Basu, S ;
Wickstrom, E .
BIOCONJUGATE CHEMISTRY, 1997, 8 (04) :481-488
[6]   Positive and negative regulation of endogenous genes by designed transcription factors [J].
Beerli, RR ;
Dreier, B ;
Barbas, CF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (04) :1495-1500
[7]   Enhanced peptide nucleic acid binding to supercoiled DNA: Possible implications for DNA ''breathing'' dynamics [J].
Bentin, T ;
Nielsen, PE .
BIOCHEMISTRY, 1996, 35 (27) :8863-8869
[8]   Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence [J].
Boffa, LC ;
Morris, PL ;
Carpaneto, EM ;
Louissaint, M ;
Allfrey, VG .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (22) :13228-13233
[9]  
Boffa LC, 2000, CANCER RES, V60, P2258
[10]   ISOLATION OF ACTIVE GENES CONTAINING CAG REPEATS BY DNA STRAND INVASION BY A PEPTIDE NUCLEIC-ACID [J].
BOFFA, LC ;
CARPANETO, EM ;
ALLFREY, VG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (06) :1901-1905