Product-substrate engineering by bacteria: Studies on clavaminate synthase, a trifunctional dioxygenase

被引：46

作者：

Lloyd, MD

Merritt, KD

Lee, V

Sewell, TJ

Wha-Son, B

Baldwin, JE

Schofield, CJ

Elson, SW

Baggaley, KH

Nicholson, NH

机构：

[1] Dyson Perrins Lab, Oxford OX1 3QY, England

[2] Oxford Ctr Mol Sci, Oxford OX1 3QY, England

[3] SmithKline Beecham Pharmaceut, Betchworth RH3 7AJ, Surrey, England

来源：

TETRAHEDRON | 1999年 / 55卷 / 33期

关键词：

amidines; enzyme reactions; hydroxylations;

D O I：

10.1016/S0040-4020(99)00547-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Evidence is presented that clavaminate synthase (CS) catalyses three oxidative reactions in the clavulanic acid biosynthetic pathway. The first CS catalysed step (hydroxylation) is separated from the latter two (oxidative cyclisation and desaturation) by the action of a hydrolytic enzyme, proclavaminate amidinohydrolase, which modifies (or 'mutates') the sidechain of the product of the first reaction thereby converting it into a substrate for the second CS catalysed reaction. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：10201 / 10220

页数：20

共 66 条

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