Protein expression dynamics during replicative senescence of endothelial cells studied by 2-D difference in-gel electrophoresis

被引:25
作者
Eman, Michael R.
Regan-Klapisz, Elsa
Pinkse, Martijn W. H.
Koop, Inge M.
Haverkamp, Johan
Heck, Albert J. R.
Verkleij, Arie J.
Post, Jan A.
机构
[1] Univ Utrecht, Biomembrane Inst, Dept Cellular Architecture & Dynam, NL-3584 CH Utrecht, Netherlands
[2] Univ Utrecht, Dept Biomol Mass Spectrometry, Bijvoet Ctr Biomol Res, Utrecht, Netherlands
[3] Univ Utrecht, Inst Pharmaceut Sci, Utrecht, Netherlands
[4] Unilever Res & Dev Lab, Vlaardingen, Netherlands
关键词
difference in-gel electrophoresis; endothelial cell; nuclear integrity; oxidative stress; replicative senescence;
D O I
10.1002/elps.200500746
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Endothelial senescence contributes to endothelium dysfunctionality and is thereby linked to vascular aging. A dynamic proteomic study on human umbilical vein endothelial cells, isolated from three umbilical cords, was performed. The cells were cultured towards replicative senescence and whole cell lysates were subjected to 2-D difference gel electrophoresis (DIGE). Despite the biological variability of the three independent isolations, a set of proteins was found that showed senescence-dependent expression patterns in all isolations. We focused on those proteins that showed significant changes, with a paired analysis of variance (RM-ANOVA) p-value of <= 0.05. Thirty-five proteins were identified with LC-Fourier transform MS, and functional annotation revealed that endothelial replicative senescence is accompanied by increased cellular stress, protein biosynthesis and reduction in DNA repair and maintenance. Nuclear integrity becomes affected and cytoskeletal structure is also changed. Such important changes in the cell infrastructure might accelerate endothelium dysfunctionality. This study provides biological information that will initiate studies to further unravel endothelial senescence and gain more knowledge about the consequences of this process in the in vivo situation.
引用
收藏
页码:1669 / 1682
页数:14
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