LucTrap vectors are tools to generate luciferase fusions for the quantification of transcript and protein abundance in vivo

被引:20
作者
Calderon-Villalobos, LIA
Kuhnle, C
Li, HB
Rosso, M
Weisshaar, B
Schwechheimer, C [1 ]
机构
[1] Ctr Plant Mol Biol, D-72076 Tubingen, Germany
[2] Max Planck Inst Plant Breeding Res, GABI Kat, D-50829 Cologne, Germany
[3] Univ Bielefeld, Inst Genome Res Ctr, Ctr Biotechnol, D-33615 Bielefeld, Germany
关键词
D O I
10.1104/pp.106.078097
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Proper plant growth and development strongly rely on the plant's ability to respond dynamically to signals and cues from the intra- and extracellular environment. Whereas many of these responses require specific changes at the level of gene expression, in recent years it has become increasingly clear that many plant responses are at least in part also controlled at the level of protein turnover. It is a challenge for signal transduction research to understand how distinct incoming signals are integrated to generate specific changes at the transcript or protein level. The activity of luciferase ( LUC) reporters can be detected in nondestructive qualitative and quantitative assays in vivo. Therefore, LUC reporters are particularly well suited for the detection of changes at the transcript and protein level. To the best of our knowledge, the number of plant transformation vectors for LUC fusions is very limited. In this article, we describe the LucTrap plant transformation vectors that allow generation of targeted and random transcriptional and translational fusions with the modified firefly LUC reporter LUC+. We demonstrate that LucTrap-based fusions can be used to monitor rapid changes in gene expression and protein abundance in vivo.
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页码:3 / 14
页数:12
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