Experimental analysis of the oil addition effect on mycelia and polysaccharide productions in Ganoderma lucidum submerged culture

被引：46

作者：

Huang, Hung-Chang ^{[1
]}

Chen, Chih-I ^{[1
,2
]}

Hung, Chia-Nan ^{[1
]}

Liu, Yung-Chuan ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Dept Chem Engn, Taichung 40227, Taiwan

[2] Hsiuping Inst Technol, Dept Chem Engn & Biotechnol, Dali, Taichung County, Taiwan

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2009年 / 32卷 / 02期

关键词：

Ganoderma lucidum; Polysaccharide; Submerged culture; Oil addition; EXOPOLYSACCHARIDE PRODUCTION; ACID; FERMENTATION; GROWTH; PH;

D O I：

10.1007/s00449-008-0239-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The effect of corn oil addition on mycelium growth and polysaccharide productions in the medicinal mushroom Ganoderma lucidum was studied. The results showed that when a level of 2% corn oil was added at the beginning of culture, the biomass and polysaccharide productions reached a maximum of 12.9 and 1.038 g/L, respectively, during 13-day cultivation. The pH variation along with morphology observation in culture provided an indirect inference to the promotional effect of oil addition. Moreover, a curve fitting analysis was carried out to assay the elevated effect on biomass and exopolysaccharide productions in oil added culture. The experimental data of substrates consumption and products formation in culture with oil addition were predicted through the fitting equations obtained in single carbon source culture. The numerical results further clarified the stimulatory effects of oil addition in G. lucidum culture.

引用

页码：217 / 224

页数：8

共 23 条

[1]

Optimization of the medium composition for the submerged culture of Ganoderma lucidum by Taguchi array design and steepest ascent method [J].

Chang, MY ;

Tsai, GJ ;

Houng, JY .

ENZYME AND MICROBIAL TECHNOLOGY, 2006, 38 (3-4) :407-414

[2]

A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[3]

Anti-HIV-1 and anti-HIV-1-protease substances from Ganoderma lucidum [J].

El-Mekkawy, S ;

Meselhy, MR ;

Nakamura, N ;

Tezuka, Y ;

Hattori, M ;

Kakiuchi, N ;

Shimotohno, K ;

Kawahata, T ;

Otake, T .

PHYTOCHEMISTRY, 1998, 49 (06) :1651-1657

[4]

Effect of initial pH on production of ganoderic acid and polysaccharide by submerged fermentation of Ganoderma lucidum [J].

Fang, QH ;

Zhong, JJ .

PROCESS BIOCHEMISTRY, 2002, 37 (07) :769-774

[5]

Submerged fermentation of higher fungus Ganoderma lucidum for production of valuable bioactive metabolites-ganoderic acid and polysaccharide [J].

Fang, QH ;

Zhong, JJ .

BIOCHEMICAL ENGINEERING JOURNAL, 2002, 10 (01) :61-65

[6]

Production of polysaccharides from Ganoderma lucidum (CCRC 36041) under limitations of nutrients [J].

Hsieh, C ;

Tseng, MH ;

Liu, CJ .

ENZYME AND MICROBIAL TECHNOLOGY, 2006, 38 (1-2) :109-117

[7]

Production of polysaccharides of Ganoderma lucidum (CCRC36021) by reusing thin stillage [J].

Hsieh, C ;

Hsu, TH ;

Yang, FC .

PROCESS BIOCHEMISTRY, 2005, 40 (02) :909-916

[8]

Effect of olive oil on the production of mycelial biomass and polysaccharides of Grifola frondosa under high oxygen concentration aeration [J].

Hsieh, Chienyan ;

Liu, Chia-Jang ;

Tseng, Mei-Hua ;

Lo, Chaur-Tsuen ;

Yang, Yuan-Chang .

ENZYME AND MICROBIAL TECHNOLOGY, 2006, 39 (03) :434-439

[9]

Culture pH affects exopolysaccharide production in submerged mycelial culture of Ganoderma lucidum [J].

Kim, Hyun Mi ;

Park, Moon Ki ;

Yun, Jong Won .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2006, 134 (03) :249-262

[10]

Bistage control of pH for improving exopolysaccharide production from mycelia of Ganoderma lucidum in an air-lift fermentor [J].

Lee, KM ;

Lee, SY ;

Lee, HY .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 88 (06) :646-650

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