Novel antiviral activity of baicalein against dengue virus

被引：152

作者：

Zandi, Keivan ^{[1
]}

Teoh, Boon-Teong ^{[1
]}

Sam, Sing-Sin ^{[1
]}

Wong, Pooi-Fong ^{[2
]}

Mustafa, Mohd Rais ^{[2
]}

AbuBakar, Sazaly ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Med Microbiol, TIDREC, Kuala Lumpur, Malaysia

[2] Univ Malaya, Fac Med, Dept Pharmacol, Kuala Lumpur 50603, Malaysia

来源：

BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2012年 / 12卷

关键词：

Infectious disease; Flavivirus; Antiviral; Dengue; Flavonoid; Baicalein; IN-VITRO; REVERSE-TRANSCRIPTASE; FLAVONOIDS; REPLICATION; ANTIOXIDANT; SEROTYPE; BINDING; TYPE-1; STRAIN; DNA;

D O I：

10.1186/1472-6882-12-214

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Background: Dengue is a serious arboviral disease currently with no effective antiviral therapy or approved vaccine available. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the natural compounds, bioflavonoids derived mainly from plants are of interest because of their biological and medicinal benefits. Methods: In the present study, antiviral activity of a bioflavonoid, baicalein, was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cells using focus forming unit reduction assay and quantitative RT-PCR. Results: Baicalein inhibited DENV-2 replication in Vero cells with IC50 = 6.46 mu g/mL and SI = 17.8 when added after adsorption to the cells. The IC50 against DENV-2 was 5.39 mu g/mL and SI = 21.3 when cells were treated 5 hours before virus infection and continuously up to 4 days post infection. Baicalein exhibited direct virucidal effect against DENV-2 with IC50 = 1.55 mu g/mL and showed anti-adsorption effect with IC50 = 7.14 mu g/mL. Conclusions: Findings presented here suggest that baicalein exerts potent antiviral activity against DENV. Baicalein possesses direct virucidal activity against DENV besides its effects against dengue virus adsorption and intracellular replication of DENV-2. Baicalein, hence, should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV.

引用

页数：9

共 38 条

[31]

*WHO, 2002, DENG HAEM FEV

[32] High intracellular Zn2+ ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells [J].