Injectable extracellular matrix hydrogel developed using porcine articular cartilage

被引：61

作者：

Kwon, Jin Seon ^{[1
]}

Yoon, So Mi ^{[1
]}

Shim, Sun Woo ^{[1
]}

Park, Ji Hoon ^{[1
]}

Min, Kyung Jun ^{[2
]}

Oh, Hyun Ju ^{[1
]}

Kim, Jae Ho ^{[1
]}

Kim, Young Jick ^{[3
]}

Yoon, Jun Jin ^{[4
]}

Choi, Byung Hyune ^{[5
]}

Kim, Moon Suk ^{[1
]}

机构：

[1] Ajou Univ, Dept Mol Sci & Technol, Suwon 443749, South Korea

[2] Hongik Univ, Chem Engn & Mat Sci, Seoul 121791, South Korea

[3] Ajou Univ, Sch Med, Cell Therapy Ctr, Suwon 443759, South Korea

[4] RegenPrime Co Ltd, R&D Div, Suwon 442819, South Korea

[5] Inha Univ, Coll Med, Div Biomed & Bioengn Sci, Inchon 400103, South Korea

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2013年 / 454卷 / 01期

关键词：

Porcine cartilage powder; Injectable; Hydrogel; BSA; Protein; BOVINE SERUM-ALBUMIN; IN-VIVO; DRUG-RELEASE; PHYSICOCHEMICAL PROPERTIES; BIOMEDICAL APPLICATIONS; PEPTIDE; ENCAPSULATION; DESIGN; ACID;

D O I：

10.1016/j.ijpharm.2013.06.023

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

This work was first development of a delivery system capable of maintaining a sustained release of protein drugs at specific sites by using potentially biocompatible porcine articular cartilage. The prepared porcine articular cartilage powder (PCP) was easily soluble in phosphate-buffered saline. The PCP suspension easily entrapped bovine serum albumin-fluorescein isothiocyanate (BSA-FITC) in pharmaceutical formulations at room temperature. The aggregation of PCP and BSA-FITC was confirmed by dynamic light scattering. When the BSA-FITC-loaded PCP suspension was subcutaneously injected into rats, it gelled and formed an interconnecting three-dimensional PCP structure that allowed BSA to penetrate through it. The amount of BSA-FITC released from the PCP hydrogel was determined in rat plasma and monitored by real-time in vivo molecular imaging. The data indicated sustained release of BSA-FITC for 20 days in vivo. In addition, the PCP hydrogel induced a slight inflammatory response. In conclusion, we showed that the PCP hydrogel could serve as a minimally invasive therapeutics depot. (c) 2013 Elsevier B.V. All rights reserved.

引用

页码：183 / 191

页数：9

共 35 条

[11]

Physicochemical properties and mechanism of drug release from ethyl cellulose matrix tablets prepared by direct compression and hot-melt extrusion [J].