The mitochondrial TOM complex modulates bax-induced apoptosis in Drosophila

被引：21

作者：

Colin, J.

Garibal, J.

Mignotte, B. ^{[1
]}

Guenal, I.

机构：

[1] Univ Versailles, CNRS, Lab Genet & Biol Cellulaire, UMR 8159, F-78035 Versailles, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 379卷 / 04期

关键词：

Apoptosis; Bax; TOM complex; Drosophila; OUTER-MEMBRANE; PRECURSOR PROTEINS; CELL-DEATH; BCL-2; PATHWAYS; PERMEABILIZATION; TRANSLOCATION; COMPONENT; RELEASE;

D O I：

10.1016/j.bbrc.2008.12.176

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bax is a pro-apoptotic member of the Bcl-2 family proteins involved in the release of apoptogenic factors from mitochondria to the cytosol. Recently, it has been shown both in mammals and yeast that Bax insertion in the mitochondrial outer membrane involves at least two distinct mechanisms, one of which uses the TOM complex. Here. we show that in Drosophila, heterozygous loss of function mutations of Tom22 or Tom70, two receptors of the TOM complex, attenuates bax-induced phenotypes in vivo. These results argue that the TOM complex may be used as a mitochondrial Bax receptor in Drosophila. (C) 2009 Elsevier Inc. All rights reserved.

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页码：939 / 943

页数：5

相关论文

共 18 条

[1] TOM22, a core component of the mitochondria outer membrane protein translocation pore, is a mitochondrial receptor for the proapoptotic protein Bax [J].

Bellot, G. ;

Cartron, P. -F. ;

Er, E. ;

Oliver, L. ;

Juin, P. ;

Armstrong, L. C. ;

Bornstein, P. ;

Mihara, K. ;

Manon, S. ;

Vallette, F. M. .

CELL DEATH AND DIFFERENTIATION, 2007, 14 (04) :785-794

[2] Multiple pathways for sorting mitochondrial precursor proteins [J].

Bolender, Natalia ;

Sickmann, Albert ;

Wagner, Richard ;

Meisinger, Chris ;

Pfanner, Nikolaus .

EMBO REPORTS, 2008, 9 (01) :42-49

[3] reaper and bax initiate two different apoptotic pathways affecting mitochondria and antagonized by bcl-2 in Drosophila [J].

Brun, S ;

Rincheval, V ;

Gaumer, S ;

Mignotte, B ;

Guenal, I .

ONCOGENE, 2002, 21 (42) :6458-6470

[4] Bax inserts into the mitochondrial outer membrane by different mechanisms [J].

Cartron, Pierre-Francois ;

Bellot, Gregory ;

Oliver, Lisa ;

Grandier-Vazeille, Xavier ;

Manon, Stephen ;

Vallette, Francois M. .

FEBS LETTERS, 2008, 582 (20) :3045-3051

[5] Characterization of the novel mitochondrial protein import component, Tom34, in mammalian cells [J].

Chewawiwat, N ;

Yano, M ;

Terada, K ;

Hoogenraad, NJ ;

Mori, M .

JOURNAL OF BIOCHEMISTRY, 1999, 125 (04) :721-727

[6] How do BCL-2 proteins induce mitochondrial outer membrane permeabilization? [J].

Chipuk, Jerry E. ;

Green, Douglas R. .

TRENDS IN CELL BIOLOGY, 2008, 18 (04) :157-164

[7] An internal EELD domain facilitates mitochondrial targeting of Mcl-1 via a Tom70-dependent pathway [J].

Chou, Chiang-Hung ;

Lee, Ru-Shuo ;

Yang-Yen, Hsin-Fang .

MOLECULAR BIOLOGY OF THE CELL, 2006, 17 (09) :3952-3963

[8]

COLIN J, 2009, FRONTIERS BIOSC S, V1, P290

[9] Bcl-2 and Bax mammalian regulators of apoptosis are functional in Drosophila [J].

Gaumer, S ;

Guénal, I ;

Brun, S ;

Théodore, L ;

Mignotte, B .

CELL DEATH AND DIFFERENTIATION, 2000, 7 (09) :804-814

[10] Drob-1, a Drosophila member of the Bcl-2/CED-9 family that promotes cell death [J].

Igaki, T ;

Kanuka, H ;

Inohara, N ;

Sawamoto, K ;

Núñez, G ;

Okano, H ;

Miura, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (02) :662-667