Zebrafish Jak2a plays a crucial role in definitive hematopoiesis and blood vessel formation

被引：6

作者：

Sung, Jin Jea ^{[1
]}

Jeon, Jinseon ^{[1
]}

Lee, Jong Joo ^{[1
]}

Kim, Chul Geun ^{[1
]}

机构：

[1] Hanyang Univ, Coll Nat Sci, Dept Life Sci, Seoul 133791, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 378卷 / 03期

关键词：

Zebrafish; Jak2a; Hematopoiesis; Blood vessel formation; shRNA; Gene knockdown; LYMPHOID DEVELOPMENT; MUTATION; PROTEIN; PATHWAY;

D O I：

10.1016/j.bbrc.2008.11.116

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

We examined the role of zebrafish (Danio rerio) Jak2a, a homolog of mammalian Jak2. in the developing embryo by injecting in vitro synthesized Jak2a shRNA into zebrafish zygotes. Blood circulation was suppressed in Jak2a shRNA-injected embryos from 24 hours post fertilization (hpf) and all embryos died with enlarged pericardium, shortened body lengths, and defects in some vasculature within 8 days post fertilization. O-dianisidine staining of Fed blood cells revealed normal blood island formation with no circulating red blood cells. As in Jak2(-/-) transgenic mice, expression of definitive Ba-1 globin was significantly reduced in Jak2a knockdown embryos at 36 hpf, whereas expression of other hematopoietic markets, primitive bel globin, gata-1, and scl, were unaffected. More importantly, blood vessel formation was disturbed in Jak2a knockdown embryos as revealed by alkaline phosphatase staining at 72 hpf. Thus, our data indicate that zebrafish Jak2a is important in both definitive hematopoiesis and blood vessel formation. (C) 2008 Elsevier Inc. All rights reserved

引用

页码：629 / 633

页数：5

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