Production of nuclear transfer-derived swine that express the enhanced green fluorescent protein

被引:164
作者
Park, KW
Cheong, HT
Lai, LX
Im, GS
Kühholzer, B
Bonk, A
Samuel, M
Rieke, A
Day, BN
Murphy, CN
Carter, DB
Prather, RS
机构
[1] Univ Missouri, Dept Anim Sci, Columbia, MO 65211 USA
[2] Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA
[3] Kangwon Natl Univ, Coll Anim Resource Sci, Dept Vet Med, Chunchon 200701, South Korea
[4] Natl Livestock Res Inst, Suwon 441350, South Korea
[5] Agrobiogen GmbH, D-86567 Hilgertshausen, Germany
关键词
D O I
10.1081/ABIO-100108344
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The ability to add or delete specific genes in swine will likely provide considerable benefits not just to agriculture but also to medicine, where pigs have potential as models for human disease and as organ donors. Here we have transferred nuclei from a genetically modified fibroblast cell line to porcine oocytes, matured in vitro under defined culture conditions, to create piglets expressing enhanced green fluorescent protein. The nuclear transfer-derived piglets were of normal size, although some mild symptoms of "large offspring syndrome" were evident. These experiments represent a next step towards creating swine with more useful genetic modifications.
引用
收藏
页码:173 / +
页数:10
相关论文
共 19 条
[1]   Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor [J].
Abeydeera, LR ;
Wang, WH ;
Cantley, TC ;
Rieke, A ;
Murphy, CN ;
Prather, RS ;
Day, BN .
THERIOGENOLOGY, 2000, 54 (05) :787-797
[2]   Production of cloned pigs from in vitro systems [J].
Betthauser, J ;
Forsberg, E ;
Augenstein, M ;
Childs, L ;
Eilertsen, K ;
Enos, J ;
Forsythe, T ;
Golueke, P ;
Jurgella, G ;
Koppang, R ;
Lesmeister, T ;
Mallon, K ;
Mell, G ;
Misica, P ;
Pace, M ;
Pfister-Genskow, M ;
Strelchenko, N ;
Voelker, G ;
Watt, S ;
Thompson, S ;
Bishop, M .
NATURE BIOTECHNOLOGY, 2000, 18 (10) :1055-1059
[3]   Transgenic monkeys produced by retroviral gene transfer into mature oocytes [J].
Chan, AWS ;
Chong, KY ;
Martinovich, C ;
Simerly, C ;
Schatten, G .
SCIENCE, 2001, 291 (5502) :309-312
[4]   Postnatal characteristics of calves produced by nuclear transfer cloning [J].
Garry, FB ;
Adams, R ;
McCann, JP ;
Odde, KG .
THERIOGENOLOGY, 1996, 45 (01) :141-152
[5]   The use of tolerance for transplantation across xenogeneic barriers [J].
Greenstein, JL ;
Sachs, DH .
NATURE BIOTECHNOLOGY, 1997, 15 (03) :235-238
[6]   In vitro produced and cloned embryos: Effects on pregnancy, parturition and offspring [J].
Kruip, TAM ;
denDaas, JHG .
THERIOGENOLOGY, 1997, 47 (01) :43-52
[7]   Six cloned calves produced from adult fibroblast cells after long-term culture [J].
Kubota, C ;
Yamakuchi, H ;
Todoroki, J ;
Mizoshita, K ;
Tabara, N ;
Barber, M ;
Yang, XZ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (03) :990-995
[8]  
KUHHOLZER B, 2001, IN PRESS BIOL REPROD
[9]   Pig cloning by microinjection of fetal fibroblast nuclei [J].
Onishi, A ;
Iwamoto, M ;
Akita, T ;
Mikawa, S ;
Takeda, K ;
Awata, T ;
Hanada, H ;
Perry, ACF .
SCIENCE, 2000, 289 (5482) :1188-1190
[10]  
PETTERS RM, 1993, J REPROD FERTIL, P61