Nitrogen catabolite repression in Saccharomyces cerevisiae during wine fermentations

被引：168

作者：

Beltran, G ^{[1
]}

Novo, M ^{[1
]}

Rozès, N ^{[1
]}

Mas, A ^{[1
]}

Guillamón, JM ^{[1
]}

机构：

[1] Univ Rovira & Virgili, Fac Enol Tarragona, Unitat Enol, Ctr Referencia Tecnol Aliments,Dept Bioquim & Biot, Tarragona 43005, Spain

来源：

FEMS YEAST RESEARCH | 2004年 / 4卷 / 06期

关键词：

wine yeast; alcoholic fermentation; nitrogen consumption; ammonium; amino acids; GAP1; MEP genes;

D O I：

10.1016/j.femsyr.2003.12.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We carried out fermentations with several nitrogen sources in different concentrations and studied nitrogen regulation by following the transcriptional profile of the general amino-acid permease (GAPI) and the ammonium permeases (MEP1, MEP2, MEP3). In wine fermentations the cells evolve from a nitrogen-repressed situation at the beginning of the process to a nitrogen-derepressed situation as the nitrogen is consumed. These nitrogen-repressed/derepressed conditions determined the different patterns of ammonium and amino-acid consumption. Arginine and alanine were hardly used under the repressed conditions, while the uptake of branched-chain and aromatic amino acids increased. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：625 / 632

页数：8

共 15 条

[1]

Aerny J, 1997, REV SUISSE VITIC ARB, V28, P161

[2] Influence of the nitrogen source on Saccharomyces cerevisiae anaerobic growth and product formation [J].

Albers, E ;

Larsson, C ;

Liden, G ;

Niklasson, C ;

Gustafsson, L .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (09) :3187-3195

[3] Arginase activity is a useful marker of nitrogen limitation during alcoholic fermentations [J].

Carrasco, P ;

Pérez-Ortín, JE ;

del Olmo, ML .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 2003, 26 (03) :471-479

[4]

CHINARD FP, 1952, J BIOL CHEM, V199, P91

[5] Sensors of extracellular nutrients in Saccharomyces cerevisiae [J].

Forsberg, H ;

Ljungdahl, PO .

CURRENT GENETICS, 2001, 40 (02) :91-109

[6]

HENSCHKE PA, 1991, PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON NITROGEN IN GRAPES AND WINE, P172

[7]

Magasainik B., 1992, MOL CELLULAR BIOL YE, V2, P283

[8] A family of ammonium transporters in Saccharomyces cerevisiae [J].

Marini, AM ;

SoussiBoudekou, S ;

Vissers, S ;

Andre, B .

MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (08) :4282-4293

[9] Transcriptional profiling of wine yeast in fermenting grape juice: regulatory effect of diammonium phosphate [J].

Marks, VD ;

van der Merwe, GK ;

van Vuuren, HJJ .

FEMS YEAST RESEARCH, 2003, 3 (03) :269-287

[10] Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae [J].

Regenberg, B ;

Düring-Olsen, L ;

Kielland-Brandt, MC ;

Holmberg, S .

CURRENT GENETICS, 1999, 36 (06) :317-328

← 1 2 →