New fluorescent labels: 4-and 7-chlorofluorescein

被引：11

作者：

Ge, FY ^{[1
]}

Yan, XL ^{[1
]}

Yan, FY ^{[1
]}

Pan, HY ^{[1
]}

Chen, LG ^{[1
]}

机构：

[1] Tianjin Univ, Sch Pharmaceut & Biotechnol, Tianjin 300072, Peoples R China

来源：

JOURNAL OF FLUORESCENCE | 2005年 / 15卷 / 06期

关键词：

fluorescent probe; 4(7)-chlorofluorescein; synthesis; fluorescent propetities;

D O I：

10.1007/s10895-005-0016-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new mixture of 4- and 7-chlorofluorescein were synthesized by condensation of resorcinol with 3-chlorophthalic anhydride in the presence of methanesulfonic acid or zinc chloride. These regioisomers were Successfully separated by chromatography. ne photophysical properties were examined and their absorption and emission maxima at long wavelength, high fluorescence quantum yield, and narrow emission bandwidth were found, highly favorable for detecting multiple target substances in the same sample. Furthermore, 4(7)-chlorofluorescein were found to be strongly pH-dependent between 4.0 and 8.0, and could be used as pH-sensitive fluorescent probe to measure intracellular pH.

引用

页码：829 / 833

页数：5