Human telomeres have different overhang sizes at leading versus lagging strands

被引:93
作者
Chai, WH [1 ]
Du, Q [1 ]
Shay, JW [1 ]
Wright, WE [1 ]
机构
[1] Univ Texas, SW Med Ctr, Dept Cell Biol, Dallas, TX 75390 USA
关键词
D O I
10.1016/j.molcel.2005.12.004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
G-rich 3' telomeric overhangs are required both for forming the distinct telomere structures to protect chromosome ends and for extending telomeres by telomerase. However, little is known about the molecular mechanisms generating telomere overhangs in human cells. We show here that cultured normal human diploid cells have longer G overhangs at telomeres generated by lagging-strand synthesis than by leading-strand synthesis. We also demonstrate that telomerase expression results in elongated overhangs at the leading daughter telomeres. Thus, the overhangs at the leading and lagging daughter telomeres are generated differently in human cells, and telomerase may preferentially affect overhangs generated at the telomeres produced by leading-strand synthesis.
引用
收藏
页码:427 / 435
页数:9
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