Analysis of betamethasone, dexamethasone and related compounds by liquid chromatography/electrospray mass spectrometry

被引：67

作者：

Arthur, KE ^{[1
]}

Wolff, JC ^{[1
]}

Carrier, DJ ^{[1
]}

机构：

[1] GlaxoSmithKline Pharmaceut, Med Res Ctr, Stevenage SG1 2NY, Herts, England

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2004年 / 18卷 / 06期

关键词：

D O I：

10.1002/rcm.1386

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatography/electrospray ionisation mass spectrometry (HPLC/ESI-MS) method has been developed to conclusively differentiate the epimers betamethasone and dexamethasone and various esterification products (betamethasone and dexamethasone 21-acetate, betamethasone and dexamethasone 21-phosphate, betamethasone 17-valerate, betamethasone 21-valerate and betamethasone 17,21-dipropionate) in counterfeit drugs. Good separation with baseline resolution of all epimers or isomers was obtained on a Zorbax Eclipse XDB or Luna C8 column, using a step gradient with mobile phases of 0.05 M ammonium acetate and acetonitrile. Betamethasones can also be distinguished by the relative abundance of their m/z 279 ion in the positive electrospray tandem mass spectra. The LC/MS or LC/MS/MS method developed was successfully applied to the analysis of drug product samples, i.e. creams and tablets. Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：678 / 684

页数：7

共 17 条

[1] Antignac JP, 2000, RAPID COMMUN MASS SP, V14, P33, DOI 10.1002/(SICI)1097-0231(20000115)14:1<33::AID-RCM829>3.3.CO
[2] 2-I
[3] Analysis of corticosteroids in hair by Liquid chromatography-electrospray ionization mass spectrometry
Bévalot, F
Gaillard, Y
Lhermitte, MA
Pépin, G
[J]. JOURNAL OF CHROMATOGRAPHY B, 2000, 740 (02): : 227 - 236
[4] STEREOCHEMICAL ANALYSIS OF BETAMETHASONE AND DEXAMETHASONE BY DERIVATIZATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
CHEN, SH
WU, SM
WU, HL
[J]. JOURNAL OF CHROMATOGRAPHY, 1992, 595 (1-2): : 203 - 208
[5] Identification of ten corticosteroids in human hair by liquid chromatography-ionspray mass spectrometry
Cirimele, V
Kintz, P
Dumestre, V
Goullé, JP
Ludes, B
[J]. FORENSIC SCIENCE INTERNATIONAL, 2000, 107 (1-3) : 381 - 388
[6] Differentiation between dexamethasone and betamethasone in a mixture using multiple mass spectrometry
De Wasch, K
De Brabander, HF
Van de Wiele, M
Vercammen, J
Courtheyn, D
Impens, S
[J]. JOURNAL OF CHROMATOGRAPHY A, 2001, 926 (01) : 79 - 86
[7] Validation of a screening method for corticosteroids in doping analysis by liquid chromatography/tandem mass spectrometry
Deventer, K
Delbeke, FT
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2003, 17 (18) : 2107 - 2114
[8] Identification of main corticosteroids as illegal feed additives in milk replacers by liquid chromatography atmospheric pressure chemical ionisation mass spectrometry
Fiori, M
Pierdominici, E
Longo, F
Brambilla, G
[J]. JOURNAL OF CHROMATOGRAPHY A, 1998, 807 (02) : 219 - 227
[9] Method for confirmation of synthetic corticosteroids in doping urine samples by liquid chromatography-electrospray ionisation mass spectrometry
Fluri, K
Rivier, L
Dienes-Nagy, A
You, CH
Maître, A
Schweizer, C
Saugy, M
Mangin, P
[J]. JOURNAL OF CHROMATOGRAPHY A, 2001, 926 (01) : 87 - 95
[10] Sensitive liquid chromatographic/tandem mass spectrometric method for the determination of beclomethasone dipropionate and its metabolites in equine plasma and urine
Guan, FY
Uboh, C
Soma, L
Hess, A
Luo, Y
Tsang, DS
[J]. JOURNAL OF MASS SPECTROMETRY, 2003, 38 (08): : 823 - 838

← 1 2 →