RCA Strands as Scaffolds To Create Nanoscale Shapes by a Few Staple Strands

被引：60

作者：

Ma, Yinzhou ^{[1
]}

Zheng, Hongning ^{[1
]}

Wang, Cuie ^{[1
]}

Yan, Qin ^{[1
]}

Chao, Jie ^{[2
]}

Fan, Chunhai ^{[2
]}

Xiao, Shou-Jun ^{[1
,3
]}

机构：

[1] Nanjing Univ, Nanjing Natl Lab Microstruct, Sch Chem & Chem Engn, State Key Lab Coordinat Chem, Nanjing 210093, Jiangsu, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China

[3] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 08期

关键词：

ROLLING CIRCLE AMPLIFICATION; DNA ORIGAMI; DESIGN;

D O I：

10.1021/ja400102s

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Thousands of nucleotide(nt)-long single strand DNAs, generated from rolling-circle-amplification (RCA), were used as scaffolds to create DNA nanoscale wires and plates with a few short staple strands by following the origami design principle with a crossover at 1.5 turns. The core sequence of the circle template, for producing tens and hundreds of tandemly repeated copies of it by RCA, was designed according to Seeman's sequence design principle for nucleic acid structural engineering (Seeman, N. C. J. Biomol. Struct. Dyn. 1990, 8, 573). The significance for folding the RCA products into nanoscale shapes lies in the design flexibility of both staple and scaffold strand codes, simplicity of a few short staple strands to fold the periodic sequence of RCA products, and lower cost.

引用

页码：2959 / 2962

页数：4

共 19 条

[1] Periodic DNA nanotemplates synthesized by rolling circle amplification [J].