Existence of uncoupling protein-2 antigen in isolated mitochondria from various tissues

被引：12

作者：

Jezek, P

Zácková, M

Reháková, Z

Ruzicka, M

Borecky, J

Skobisová, E

Brucknerová, J

Garlid, KD

Gimeno, RE

Tartaglia, LA

机构：

[1] Inst Physiol, Dept Membrane Transport Biophys, CZ-14220 Prague, Czech Republic

[2] Inst Microbiol, Dept Gnotobiol, Novy Hradek, Czech Republic

[3] Oregon Grad Inst Sci & Technol, Dept Biochem & Mol Biol, Portland, OR 97291 USA

[4] Millennium Pharmaceut Inc, Cambridge, MA 02139 USA

来源：

FEBS LETTERS | 1999年 / 455卷 / 1-2期

关键词：

anti-UCP3; antibody; uncoupling protein 2; uncoupling protein 3;

D O I：

10.1016/S0014-5793(99)00853-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies against Escherichia coli-expressed uncoupling protein-2 (UCP2) and uncoupling protein-3 (UCP3) were raised by operating the blotted proteins into the spleen of minipigs, The antisera reacted more intensively with the recombinant UCP2 and UCP3 than with uncoupling protein-1 (UCP1) isolated from brown adipose tissue. Moreover, anti-UCP2 and cross-reacting anti-UCP3 antibodies identified the presence of the UCP2/3 antigen in isolated mitochondria from rat heart, rat kidney, rat brain, rabbit epididymal white adipose tissue, hamster brown adipose tissue, and rabbit skeletal muscle. It has been concluded that UCP2 is expressed in these tissues (UCP3 in skeletal muscle); however their existence in mitochondria had not previously been demonstrated. (C) 1999 Federation of European Biochemical Societies.

引用

页码：79 / 82

页数：4

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