Direct and indirect lactate oxidation in trained and untrained men

被引:48
作者
Emhoff, Chi-An W. [1 ]
Messonnier, Laurent A. [1 ,2 ]
Horning, Michael A. [1 ]
Fattor, Jill A. [1 ]
Carlson, Thomas J. [1 ]
Brooks, George A. [1 ]
机构
[1] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA
[2] Univ Savoie, Lab Physiol Exercice, Le Bourget Du Lac, France
关键词
lactate oxidation; gluconeogenesis; glucose; muscle glycogen; exercise; training; stable isotope tracers; HUMAN SKELETAL-MUSCLE; MODERATE-INTENSITY EXERCISE; GLYCOGEN-SYNTHESIS; GLUCOSE KINETICS; MAXIMAL EXERCISE; LEG EXERCISE; LACTIC-ACID; RAT-HEART; IN-VIVO; ENDURANCE;
D O I
10.1152/japplphysiol.00538.2013
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Lactate has been shown to be an important oxidative fuel. We aimed to quantify the total lactate oxidation rate (R-ox) and its direct vs. indirect (glucose that is gluconeogenically derived from lactate and subsequently oxidized) components (mg.kg(-1).min(-1)) during rest and exercise in humans. We also investigated the effects of endurance training, exercise intensity, and blood lactate concentration ([lactate](b)) on direct and indirect lactate oxidation. Six untrained (UT) and six trained (T) men completed 60 min of constant load exercise at power outputs corresponding to their lactate threshold (LT). T subjects completed two additional 60-min sessions of constant load exercise at 10% below the LT workload (LT - 10%), one of which included a lactate clamp (LC; LT - 10% + LC). R-ox was higher at LT in T [22.7 +/- 2.9, 75% peak oxygen consumption ((V)O-2peak)] compared with UT (13.4 +/- 2.5, 68% (V)O-2peak, P < 0.05). Increasing [lactate] b (LT - 10% + LC, 67% (V)O-2peak) significantly increased lactate R-ox (27.9 +/- 3.0) compared with its corresponding LT - 10% control (15.9 +/- 2.2, P < 0.05). Direct and indirect R-ox increased significantly from rest to exercise, and their relative partitioning remained constant in all trials but differed between T and UT: direct oxidation comprised 75% of total lactate oxidation in UT and 90% in T, suggesting the presence of training-induced adaptations. Partitioning of total carbohydrate (CHO) use showed that subjects derived one-third of CHO energy from blood lactate, and exogenous lactate infusion increased lactate oxidation significantly, causing a glycogen-sparing effect in exercising muscle.
引用
收藏
页码:829 / 838
页数:10
相关论文
共 86 条
[61]  
MCLANE JA, 1979, J BIOL CHEM, V254, P6548
[62]   Lactate exchange and removal abilities in rowing performance [J].
Messonnier, L ;
Freund, H ;
Bourdin, M ;
Belli, A ;
Lacour, JR .
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 1997, 29 (03) :396-401
[63]   Lactate kinetics at the lactate threshold in trained and untrained men [J].
Messonnier, Laurent A. ;
Emhoff, Chi-An W. ;
Fattor, Jill A. ;
Horning, Michael A. ;
Carlson, Thomas J. ;
Brooks, George A. .
JOURNAL OF APPLIED PHYSIOLOGY, 2013, 114 (11) :1593-1602
[64]   Lactate and glucose interactions during rest and exercise in men: effect of exogenous lactate infusion [J].
Miller, BF ;
Fattor, JA ;
Jacobs, KA ;
Horning, MA ;
Navazio, F ;
Lindinger, MI ;
Brooks, GA .
JOURNAL OF PHYSIOLOGY-LONDON, 2002, 544 (03) :963-975
[65]   Metabolic and cardiorespiratory responses to "the lactate clamp" [J].
Miller, BF ;
Fattor, JA ;
Jacobs, KA ;
Horning, MA ;
Suh, SH ;
Navazio, F ;
Brooks, GA .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2002, 283 (05) :E889-E898
[66]   Hepatic lactate uptake versus leg lactate output during exercise in humans [J].
Nielsen, H. B. ;
Febbraio, M. A. ;
Ott, P. ;
Krustrup, P. ;
Secher, N. H. .
JOURNAL OF APPLIED PHYSIOLOGY, 2007, 103 (04) :1227-1233
[67]   Impact of carbohydrate supplementation during endurance training on glycogen storage and performance [J].
Nybo, L. ;
Pedersen, K. ;
Christensen, B. ;
Aagaard, P. ;
Brandt, N. ;
Kiens, B. .
ACTA PHYSIOLOGICA, 2009, 197 (02) :117-127
[68]   ROLE OF CELL TYPE IN NET LACTATE REMOVAL BY SKELETAL-MUSCLE [J].
PAGLIASSOTTI, MJ ;
DONOVAN, CM .
AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 258 (04) :E635-E642
[69]   Effects of training duration on substrate turnover and oxidation during exercise [J].
Phillips, SM ;
Green, HJ ;
Tarnopolsky, MA ;
Heigenhauser, GJF ;
Hill, RE ;
Grant, SM .
JOURNAL OF APPLIED PHYSIOLOGY, 1996, 81 (05) :2182-2191
[70]   INDOCYANINE GREEN CLEARANCE + ESTIMATED HEPATIC BLOOD FLOW DURING MILD TO MAXIMAL EXERCISE IN UPRIGHT MAN [J].
ROWELL, LB ;
BRUCE, RA ;
BLACKMON, JR .
JOURNAL OF CLINICAL INVESTIGATION, 1964, 43 (08) :1677-&