Direct and indirect lactate oxidation in trained and untrained men

被引:48
作者
Emhoff, Chi-An W. [1 ]
Messonnier, Laurent A. [1 ,2 ]
Horning, Michael A. [1 ]
Fattor, Jill A. [1 ]
Carlson, Thomas J. [1 ]
Brooks, George A. [1 ]
机构
[1] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA
[2] Univ Savoie, Lab Physiol Exercice, Le Bourget Du Lac, France
关键词
lactate oxidation; gluconeogenesis; glucose; muscle glycogen; exercise; training; stable isotope tracers; HUMAN SKELETAL-MUSCLE; MODERATE-INTENSITY EXERCISE; GLYCOGEN-SYNTHESIS; GLUCOSE KINETICS; MAXIMAL EXERCISE; LEG EXERCISE; LACTIC-ACID; RAT-HEART; IN-VIVO; ENDURANCE;
D O I
10.1152/japplphysiol.00538.2013
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Lactate has been shown to be an important oxidative fuel. We aimed to quantify the total lactate oxidation rate (R-ox) and its direct vs. indirect (glucose that is gluconeogenically derived from lactate and subsequently oxidized) components (mg.kg(-1).min(-1)) during rest and exercise in humans. We also investigated the effects of endurance training, exercise intensity, and blood lactate concentration ([lactate](b)) on direct and indirect lactate oxidation. Six untrained (UT) and six trained (T) men completed 60 min of constant load exercise at power outputs corresponding to their lactate threshold (LT). T subjects completed two additional 60-min sessions of constant load exercise at 10% below the LT workload (LT - 10%), one of which included a lactate clamp (LC; LT - 10% + LC). R-ox was higher at LT in T [22.7 +/- 2.9, 75% peak oxygen consumption ((V)O-2peak)] compared with UT (13.4 +/- 2.5, 68% (V)O-2peak, P < 0.05). Increasing [lactate] b (LT - 10% + LC, 67% (V)O-2peak) significantly increased lactate R-ox (27.9 +/- 3.0) compared with its corresponding LT - 10% control (15.9 +/- 2.2, P < 0.05). Direct and indirect R-ox increased significantly from rest to exercise, and their relative partitioning remained constant in all trials but differed between T and UT: direct oxidation comprised 75% of total lactate oxidation in UT and 90% in T, suggesting the presence of training-induced adaptations. Partitioning of total carbohydrate (CHO) use showed that subjects derived one-third of CHO energy from blood lactate, and exogenous lactate infusion increased lactate oxidation significantly, causing a glycogen-sparing effect in exercising muscle.
引用
收藏
页码:829 / 838
页数:10
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