Statistical optimization of enzymatic synthesis of derivatives of trehalose and sucrose

被引:17
作者
Smith, NK
Gilmour, SG
Rastall, RA
机构
[1] UNIV READING,DEPT FOOD SCI & TECHNOL,BIOTECHNOL & BIOCHEM ENGN GRP,READING RG6 6AP,BERKS,ENGLAND
[2] UNIV READING,DEPT APPL STAT,READING RG6 6AP,BERKS,ENGLAND
关键词
D O I
10.1016/S0141-0229(97)00004-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The synthesis of the oligosaccharides 6-O-alpha-mannosyltrehalose, 6-O-alpha-galactosyltrehalose, 6-O-alpha-galactosyltrehalose, and 6-O-g-alpha-mannosylsucrose, using appropriate glycosidases was optimized using response surface methodology.(1) An empirical mathematical model was constructed to predict the amount of product formed under various conditions of pH, substrate concentration, and donor:acceptor ratio. (C) 1997 Elsevier Science Inc.
引用
收藏
页码:349 / 354
页数:6
相关论文
共 9 条