Highly sensitive biomolecular fluorescence detection using nanoscale ZnO platforms

被引:162
作者
Dorfman, Adam [1 ]
Kumar, Nitin [1 ]
Hahm, Jong-in [1 ]
机构
[1] Penn State Univ, Dept Chem Engn, Fenske Lab 160, University Pk, PA 16802 USA
关键词
D O I
10.1021/la053270+
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Fluorescence detection is currently one of the most widely used methods in the areas of basic biological research, biotechnology, cellular imaging, medical testing, and drug discovery. Using model protein and nucleic acid systems, we demonstrate that engineered nanoscale zinc oxide structures can significantly enhance the detection capability of biomolecular fluorescence. Without any chemical or biological amplification processes, nanoscale zinc oxide platforms enabled increased fluorescence detection of these biomolecules when compared to other commonly used substrates such as glass, quartz, polymer, and silicon. The use of zinc oxide nanorods as fluorescence enhancing substrates in our biomolecular detection permitted sub-picomolar and attomolar detection sensitivity of proteins and DNA, respectively, when using a conventional fluorescence microscope. This ultrasensitive detection was due to the presence of ZnO nanomaterials which contributed greatly to the increased signal-to-noise ratio of biomolecular fluorescence. We also demonstrate the easy integration potential of zinc oxide nanorods into periodically patterned nanoplatforms which, in turn, will promote the assembly and fabrication of these materials into multiplexed, high-throughput, optical sensor arrays. These zinc oxide nanoplatforms will be extremely beneficial in accomplishing highly sensitive and specific detection of biological samples involving nucleic acids, proteins and cells, particularly under detection environments involving extremely small sample volumes of ultratrace-level concentrations.
引用
收藏
页码:4890 / 4895
页数:6
相关论文
共 36 条
  • [1] Identification of a region of genetic variability among Bacillus anthracis strains and related species
    Andersen, GL
    Simchock, JM
    Wilson, KH
    [J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (02) : 377 - 384
  • [2] Metal-enhanced fluorescence solution-based sensing platform
    Aslan, K
    Lakowicz, JR
    Szmacinski, H
    Geddes, CD
    [J]. JOURNAL OF FLUORESCENCE, 2004, 14 (06) : 677 - 679
  • [3] Enhanced fluorescence cyanide detection at physiologically lethal levels: Reduced ICT-based signal transduction
    Badugu, R
    Lakowicz, JR
    Geddes, CD
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (10) : 3635 - 3641
  • [4] Measuring the work function at a nanobelt tip and at a nanoparticle surface
    Bai, XD
    Wang, EG
    Gao, PX
    Wang, ZL
    [J]. NANO LETTERS, 2003, 3 (08) : 1147 - 1150
  • [5] Quantum dots in biology and medicine
    Bailey, RE
    Smith, AM
    Nie, SM
    [J]. PHYSICA E-LOW-DIMENSIONAL SYSTEMS & NANOSTRUCTURES, 2004, 25 (01) : 1 - 12
  • [6] DETECTION OF SINGLE RHODAMINE-6G MOLECULES IN LEVITATED MICRODROPLETS
    BARNES, MD
    NG, KC
    WHITTEN, WB
    RAMSEY, JM
    [J]. ANALYTICAL CHEMISTRY, 1993, 65 (17) : 2360 - 2365
  • [7] EXPERIMENTAL STUDIES ON SPECTRAL SENSITIZATION OF PHOTOCONDUCTIVITY ON ZINC-OXIDE CRYSTALS
    BAUER, W
    [J]. JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS, 1977, 38 (05) : 463 - 467
  • [8] Single-mismatch detection using gold-quenched fluorescent oligonucleotides
    Dubertret, B
    Calame, M
    Libchaber, AJ
    [J]. NATURE BIOTECHNOLOGY, 2001, 19 (04) : 365 - 370
  • [9] Integrated capillary electrophoresis on flexible silicone microdevices: Analysis of DNA restriction fragments and detection of single DNA molecules on microchips
    Effenhauser, CS
    Bruin, GJM
    Paulus, A
    Ehrat, M
    [J]. ANALYTICAL CHEMISTRY, 1997, 69 (17) : 3451 - 3457
  • [10] SORTING SINGLE MOLECULES - APPLICATION TO DIAGNOSTICS AND EVOLUTIONARY BIOTECHNOLOGY
    EIGEN, M
    RIGLER, R
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) : 5740 - 5747