Studies on the interaction of colloidal gold and serum albumins by spectral methods

被引：111

作者：

Gao, DJ ^{[1
]}

Tian, Y ^{[1
]}

Bi, SY ^{[1
]}

Chen, YH ^{[1
]}

Yu, AM ^{[1
]}

Zhang, HQ ^{[1
]}

机构：

[1] Jilin Univ, Coll Chem, Changchun 130012, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2005年 / 62卷 / 4-5期

关键词：

fluorescence quenching; colloidal gold; serum albumins;

D O I：

10.1016/j.saa.2005.04.026

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The interactions of colloidal gold and serum albumins, including bovine serum albumin (BSA) and human serum albumin (HSA), were studied by fluorescence and absorption spectrometry. Fluorescence quenching spectrometry was applied to study the interactions between colloidal gold and serum albumins. At pH 7.4 phosphate-buffered saline (PBS), the intensity of fluorescence emission spectrum of serum albumins decreased in the presence of colloidal gold, which indicated that colloidal gold quenched the fluorescence of serum albumins. Experimental results indicated that the combination reactions of colloidal gold and serum albumins were static quenching processes. Based on the effect of colloidal gold on fluorescence intensity, the binding constants, the numbers of binding sites and the acting forces between colloidal gold and serum albumins were found. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：1203 / 1208

页数：6

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