Development of a reverse genetics system to generate a recombinant Ebola virus Makona expressing a green fluorescent protein

被引：41

作者：

Albarino, Cesar G. ^{[1
]}

Guerrero, Lisa Wiggleton ^{[1
]}

Lo, Michael K. ^{[1
]}

Nichol, Stuart T. ^{[1
]}

Towner, Jonathan S. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Atlanta, GA 30322 USA

来源：

VIROLOGY | 2015年 / 484卷

关键词：

DEPENDENT RNA-POLYMERASE; HEMORRHAGIC-FEVER; MARBURG VIRUS; ANTIVIRAL COMPOUNDS; IRF-3; ACTIVATION; DISEASE OUTBREAK; HIGH-THROUGHPUT; WEST-AFRICA; IN-VITRO; REPLICATION;

D O I：

10.1016/j.virol.2015.06.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Previous studies have demonstrated the potential application of reverse genetics technology in studying a broad range of aspects of viral biology, including gene regulation, protein function, cell entry, and pathogenesis. Here, we describe a highly efficient reverse genetics system used to generate recombinant Ebola virus (EBOV) based on a recent isolate from a human patient infected during the 2014-2015 outbreak in Western Africa. We also rescued a recombinant EBOV expressing a fluorescent reporter protein from a cleaved VP40 protein fusion. Using this virus and an inexpensive method to quantitate the expression of the foreign gene, we demonstrate its potential usefulness as a tool for screening antiviral compounds and measuring neutralizing antibodies. Published by Elsevier Inc.

引用

页码：259 / 264

页数：6

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