Detection of Ob-receptor in human adrenal neoplasms and effect of leptin on adrenal cell proliferation

被引:39
作者
Glasow, A
Bornstein, SR
Chrousos, GP
Brown, JW
Scherbaum, WA
机构
[1] Univ Leipzig, Med Klin & Poliklin 3, Dept Internal Med 3, D-04103 Leipzig, Germany
[2] NICHHD, Dev Endocrinol Branch, NIH, Bethesda, MD 20892 USA
[3] Univ Miami, Sch Med, Dept Med, Miami, FL USA
[4] US Dept Vet Affairs, Ctr Med, Miami, FL USA
[5] Univ Dusseldorf, Diabet Res Inst, D-4000 Dusseldorf, Germany
关键词
immunostaining; leptin receptor; adrenal pathologies;
D O I
10.1055/s-2007-978726
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Leptin, a hormone mainly secreted from adipose tissue, communicates a metabolic signal to the adrenal gland. Ob-Receptor (Ob-R) expression was reported in rat, mice and human adrenal glands. This study intended to investigate possible differences in the Ob-R expression acid distribution of Ob-R protein in human adrenal tumors as compared to normal adrenal tissue. Proliferative effects of leptin were analyzed in the human adrenocortical carcinoma cell line (NCl-H295). The full length Ob-R mRNA and the isoforms B219.1 and B219.3 could be demonstrated by RT-PCR in all adrenal tumors (n=8), the tumor cell line (NCl-H295) and normal tissue. In contrast the Ob-R isoform B219.2 was absent in the carcinoma cell line and in most of the adrenal tumors (n = 5), whereas it was present in normal adrenals. The Ob-R protein could be demonstrated in benign and malignant adrenocortical tumors. Pheochromocytomas showed only a weak immunostaining with the human Ob-R antibody. Human leptin did not affect the proliferation or variability of adrenal tumor cells as demonstrated by [H-3]-thymidine assay and WST-1 test. In conclusion, although functional leptin receptors are expressed in human adrenal tumors, leptin does not regulate tumor cell proliferation.
引用
收藏
页码:247 / 251
页数:5
相关论文
共 21 条
[11]  
LACROIX A, 1992, NEW ENGL J MED, V327, P973
[12]   Effects of recombinant murine leptin on steroid secretion of dispersed rat adrenocortical cells [J].
Malendowicz, LK ;
Nussdorfer, GG ;
Markowska, A .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1997, 63 (1-3) :123-125
[13]   Dexamethasone induces an acute and sustained rise in circulating leptin levels in normal human subjects [J].
Miell, JP ;
Englaro, P ;
Blum, WF .
HORMONE AND METABOLIC RESEARCH, 1996, 28 (12) :704-707
[14]   Angiotensin II receptors in cortical and medullary adrenal tumors [J].
Opocher, G ;
Rocco, S ;
Cimolato, M ;
Vianello, B ;
Arnaldi, G ;
Mantero, F .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1997, 82 (03) :865-869
[15]   Dexamethasone increases leptin expression in humans in vivo [J].
PapaspyrouRao, S ;
Schneider, SH ;
Petersen, RN ;
Fried, SK .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1997, 82 (05) :1635-1637
[16]   Leptin inhibits directly glucocorticoid secretion by normal human and rat adrenal gland [J].
Pralong, FP ;
Roduit, R ;
Waeber, G ;
Castillo, E ;
Mosimann, F ;
Thorens, B ;
Gaillard, RC .
ENDOCRINOLOGY, 1998, 139 (10) :4264-4268
[17]   Leptin induces mitogen-activated protein kinase-dependent proliferation of C3H10T1/2 cells [J].
Takahashi, Y ;
Okimura, Y ;
Mizuno, I ;
Iida, K ;
Takahashi, T ;
Kaji, H ;
Abe, H ;
Chihara, K .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (20) :12897-12900
[18]   Leptin induces proliferation of pancreatic β cell line MIN6 through activation of mitogen-activated protein kinase [J].
Tanabe, K ;
Okuya, S ;
Tanizawa, Y ;
Matsutani, A ;
Oka, Y .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 241 (03) :765-768
[19]   Leptin stimulates the proliferation of murine myelocytic and primitive hematopoietic progenitor cells [J].
Umemoto, Y ;
Tsuji, K ;
Yang, FC ;
Ebihara, Y ;
Kaneko, A ;
Furukawa, S ;
Nakahata, T .
BLOOD, 1997, 90 (09) :3438-3443
[20]   Leptin receptor (OB-R) signaling - Cytoplasmic domain mutational analysis and evidence for receptor homo-oligomerization [J].
White, DW ;
Kuropatwinski, KK ;
Devos, R ;
Baumann, H ;
Tartaglia, LA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (07) :4065-4071