Coupling chemometrics and electrochemical-based sensor for detection of bacterial population

被引：15

作者：

Berrettoni, MB

Carpani, I

Corradini, N

Conti, P

Fumarola, G

Legnani, G

Lanteri, S

Marassi, R

Tonelli, D

机构：

[1] Univ Genoa, Dipartimento Chim & Tecnol Farmaceut Alimentari, I-16147 Genoa, Italy

[2] Univ Bologna, Dipartimento Chim Fis & Inorgan, I-40136 Bologna, Italy

[3] INSTM, UdR Bologna, I-40136 Bologna, Italy

[4] Univ Camerino, Dipartimento Sci Chim, I-62032 Camerino, Italy

[5] Univ Bologna, Dipartimento Med & Sanita Pubbl, I-40126 Bologna, Italy

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 509卷 / 01期

关键词：

PLS; SIMCA; normal pulse voltammetry; electrochemical sensor; bacterial sensor; Escherichia coli; Pseudomonas aeruginosa;

D O I：

10.1016/j.aca.2003.12.025

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

This work describes a home-made microelectrode array, based on reticulated vitreous carbon, which has been used to record the normal pulse voltammograms with the aim of obtaining the growth curves of Escherichia coli ATCC 13706 and Pseudomonas aeruginosa ATCC 27853 chosen as test microorganisms. The electrochemical signal data have been analysed with partial least squares (PLS) regression in order to highlight the useful analytical information and correlate with the data obtained from the aerobic plate counting. The obtained PLS models generally had a low root mean square error of cross-validation (RMSECV), a cross validated explained variance percentage near 90%. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：95 / 101

页数：7

共 20 条

[1]

Chemometric study and validation strategies in the structure-activity relationships of new cardiotonic agents [J].