Screen anticancer drug in vitro using resonance light scattering technique

被引：32

作者：

Chen, Zhanguang ^{[1
]}

Liu, Guoliang ^{[1
,2
]}

Chen, Meizhen ^{[2
]}

Xu, Benjie ^{[2
]}

Peng, Yurui ^{[1
]}

Chen, Maohuai ^{[3
]}

Wu, Mingyao ^{[3
]}

机构：

[1] Shantou Univ, Dept Chem, Shantou 515063, Peoples R China

[2] Shantou Univ, Dept Biol, Shantou 515063, Peoples R China

[3] Shantou Univ, Coll Med, Shantou 515041, Peoples R China

来源：

TALANTA | 2009年 / 77卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Resonance light scattering; Screen in vitro; Anticancer drug; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; Hepatocellular carcinoma cell of mouse; RAYLEIGH-SCATTERING; NUCLEIC-ACIDS; WATER/TETRACHLOROMETHANE INTERFACE; CETYLTRIMETHYLAMMONIUM BROMIDE; HEPATOCELLULAR-CARCINOMA; SILVER NANOPARTICLES; CELL-CULTURE; LUNG-CANCER; ASSAY; COMPLEX;

D O I：

10.1016/j.talanta.2008.09.016

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An in vitro screening model using resonance light scattering (RLS) technique with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reagent as the reactive probe to target cancer cell was firstly developed. In this model, MTT was reduced by viable cancer cells to produce a purple formazan. Cell viability was proportional to the number of formazan induced strong light scattering signal. The inhibition rate of anticancer drug was found to vary inversely with the H-22-MTT system RLS intensity. So it was intuitive to see the sequence of the tumor suppressive activity of six anticancer drugs without data processing by RLS/MTT screening spectra. Compared with the traditional MTT method, this method has high sensitivity, low detection limit and quite intuitive screening results which were identical to those obtained from the MTT colorimetric assay. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1365 / 1369

页数：5

共 37 条

[1] MEASUREMENT OF THE DEPOLARIZATION RATIO OF RAYLEIGH-SCATTERING AT ABSORPTION-BANDS [J].

ANGLISTER, J ;

STEINBERG, IZ .

JOURNAL OF CHEMICAL PHYSICS, 1981, 74 (02) :786-791

[2] A simple and sensitive assay of nucleic acids based on the enhanced resonance light scattering of zwitterionics [J].

Chen, ZG ;

Ding, WF ;

Ren, FL ;

Liu, JB ;

Liang, YZ .

ANALYTICA CHIMICA ACTA, 2005, 550 (1-2) :204-209

[3] Rapid and sensitive determination of proteins by enhanced resonance light scattering spectroscopy of sodium lauroyl glutamate [J].

Chen, Zhanguang ;

Liu, Jinbin ;

Han, Yali .

TALANTA, 2007, 71 (03) :1246-1251

[4] Use of sodium lauroyl sarcosinate in a high-sensitivity protein assay by resonance light scattering technique [J].

Chen, Zhanguang ;

Liu, Jinbin ;

Liang, Yizeng ;

Ren, Fenglian .

JOURNAL OF BIOMOLECULAR SCREENING, 2006, 11 (04) :400-406

[5] A novel histidine assay using tetraphenylporphyrin manganese (III) chloride as a molecular recognition probe by resonance light scattering technique [J].

Chen, Zhanguang ;

Liu, Jinbin ;

Han, Yali ;

Zhu, Li .

ANALYTICA CHIMICA ACTA, 2006, 570 (01) :109-115

[6] Total internal reflected resonance light scattering determination of protein in human blood serum at water/tetrachloromethane interface with Arsenazo-TB and Cetyltrimethylammonium bromide [J].

Dong, Lijun ;

Chen, Xingguo ;

Hu, Zhide .

TALANTA, 2007, 71 (02) :555-560

[7] Total internal reflected resonance light scattering determination of chlortetracycline in body fluid with the complex cation of chlortetracycline-europium-trioctyl phosphine oxide at the water/tetrachloromethane interface [J].

Feng, P ;

Shu, WQ ;

Huang, CZ ;

Li, YF .

ANALYTICAL CHEMISTRY, 2001, 73 (17) :4307-4312

[8] A reliable tool to determine cell viability in complex 3-D culture: The acid phosphatase assay [J].

Friedrich, Juergen ;

Eder, Wolfgang ;

Castaneda, Juana ;

Doss, Markus ;

Huber, Elisabeth ;

Ebner, Reinhard ;

Kunz-Schughart, Leoni A. .

JOURNAL OF BIOMOLECULAR SCREENING, 2007, 12 (07) :925-937

[9] INVITRO ANTITUMOR-ACTIVITY OF MITOMYCIN-C DERIVATIVE (RM-49) AND NEW ANTICANCER ANTIBIOTICS (FK973) AGAINST LUNG-CANCER CELL-LINES DETERMINED BY TETRAZOLIUM DYE (MTT) ASSAY [J].

HORIUCHI, N ;

NAKAGAWA, K ;

SASAKI, Y ;

MINATO, K ;

FUJIWARA, Y ;

NEZU, K ;

OHE, Y ;

SAIJO, N .

CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1988, 22 (03) :246-250

[10] The sulphorhodamine (SRB) assay and other approaches to testing plant extracts and derived compounds for activities related to reputed anticancer activity [J].

Houghton, Peter ;

Fang, Rui ;

Techatanawat, Isariya ;

Steventon, Glyn ;

Hylands, Peter J. ;

Lee, C. C. .

METHODS, 2007, 42 (04) :377-387

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