miRNA Stability in Frozen Plasma Samples

被引：113

作者：

Balzano, Francesca ^{[1
]}

Deiana, Marta ^{[2
]}

Dei Giudici, Silvia ^{[3
]}

Oggiano, Annalisa ^{[3
]}

Baralla, Angela ^{[1
]}

Pasella, Sara ^{[1
]}

Mannu, Andrea ^{[1
]}

Pescatori, Mario ^{[1
]}

Porcu, Baingio ^{[1
]}

Fanciulli, Giuseppe ^{[4
]}

Zinellu, Angelo ^{[1
]}

Carru, Ciriaco ^{[1
]}

Deiana, Luca ^{[1
,2
]}

机构：

[1] Univ Sassari, Dept Biomed Sci, I-07100 Sassari, Italy

[2] Assoc Isola Centenari, I-07100 Sassari, Italy

[3] Ist Zooprofilatt Sperimentale Sardegna, I-07100 Sassari, Italy

[4] Univ Sassari, Dept Clin & Expt Med, I-07100 Sassari, Italy

来源：

MOLECULES | 2015年 / 20卷 / 10期

关键词：

miRNA; stability; long freezing times; sequences AU or UA; CIRCULATING MICRORNAS; MESSENGER-RNA; BIOMARKERS; TARGETS;

D O I：

10.3390/molecules201019030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

MicroRNAs (miRNAs) represent a family of small non-coding ribonucleic acids that post-transcriptionally inhibits the expression of their target messenger RNAs (mRNAs), thereby acting as general gene repressors. In this study we examined the relative quantity and stability of miRNA subjected to a long period of freezing; we compared the stability of eight miRNAs in the plasma of five human healthy controls before freezing and after six and 12 months of storage at -80 degrees C. In addition, we examined the plasma frozen for 14 years and the amount of miRNA still available. Using a Life Technologies protocol to amplify and quantify plasma miRNAs from EDTA (Ethylene Diamine Tetraacetic Acid)-treated blood, we analyzed the stability of eight miRNAs, (miR-125b-5p, miR-425-5p, miR-200b-5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-126-3p, and miR-21-5p). The miRNAs analyzed showed a high stability and long frozen half-life.

引用

页码：19030 / 19040

页数：11

共 23 条

[1]

Aijt S.K., 2012, MOL SENS, V12, P3359

[2]

The functions of animal microRNAs [J].