Raising intracellular calcium attenuates neuronal apoptosis triggered by staurosporine or oxygen-glucose deprivation in the presence of glutamate receptor blockade

被引:19
作者
Canzoniero, LMT
Babcock, DJ
Gottron, FJ
Grabb, MC
Manzerra, P
Snider, BJ
Choi, DW
机构
[1] Washington Univ, Sch Med, Ctr Study Nervous Syst Injury, St Louis, MO 63110 USA
[2] Washington Univ, Sch Med, Dept Neurol, St Louis, MO 63110 USA
关键词
calcium; apoptosis; staurosporine;
D O I
10.1016/j.nbd.2003.10.013
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The relationship between intracellular Ca2+ ([Ca2+](i)) regulation and programmed cell death is not well-defined; both increases and decreases in [Ca2+](i) have been observed in cells undergoing apoptosis. We determined [Ca2+](i) in cultured murine cortical neurons undergoing apoptosis after exposure to staurosporine or following oxygen-glucose deprivation in the presence of glutamate receptor antagonists. Neuronal [Ca2+](i) was decreased 1-4 h after exposure to staurosporine (30 nM). A [Ca2+](i) decrease was also observed 1 h after the end of the oxygen-glucose deprivation period when MK-801 and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) were added to the bathing medium during the deprivation period. A similar decrease in [Ca2+](i) produced by reducing extracellular Ca2+ or chelating intracellular Ca2+ was sufficient to induce neuronal apoptosis. Raising [Ca2+](i) either by activating voltage-sensitive Ca2+ channels with (-) Bay K8644 or by application of low concentrations of kainate attenuated both staurosporine and oxygen-glucose deprivation-induced apoptosis. (C) 2004 Published by Elsevier Inc.
引用
收藏
页码:520 / 528
页数:9
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