G-CSF protects human cerebral hybrid neurons against in vitro ischemia

被引：27

作者：

Jung, KH

Chu, K

Lee, ST

Kang, LM

Kim, SU

Kim, M

Roh, JK ^{[1
]}

机构：

[1] Seoul Natl Univ Hosp, Dept Neurol, Stroke & Neural Stam Cell Lab Clin Res Inst, Seoul 110744, South Korea

[2] Korea Ctr Dis Control & Prevent, Dept Dis Invest & Surveillance, Seoul, South Korea

[3] Seoul Natl Hosp, Ctr Alcohol & Drug Addict Res, Seoul, South Korea

[4] Ajou Univ, Brain Dis Res Ctr, Suwon 441749, South Korea

[5] Univ British Columbia, UBC Hosp, Dept Med, Div Neurol, Vancouver, BC V5Z 1M9, Canada

来源：

NEUROSCIENCE LETTERS | 2006年 / 394卷 / 03期

关键词：

G-CSF; ischemia; neuronal death; neuroprotection; G-CSFR; STAT3;

D O I：

10.1016/j.neulet.2005.10.040

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Granulocyte colony-stimulating factor (G-CSF) protects neurons against experimental focal cerebral ischemia. However, its neuroprotective effect on human brain is unknown. We sought to determine whether G-CSF can protect the human cerebral neurons in vitro. Human cerebral-neuroblastoma hybrid cell line (A1) was exposed to oxygen and glucose deprivation with or without G-CSF. G-CSF promoted cell survival and decreased cytotoxicity effectively at 25 ng/ml. G-CSF reduced early apoptotic (annexin V+/PI-), and late apoptotic or necrotic (annexin V+/PI+) cells, and decreased active caspase-3 immunoreactivity. G-CSF could protect human cerebral neurons following in vitro ischemia. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：168 / 173

页数：6

共 25 条

[1] Stroke induces widespread changes of gene expression for glial cell line-derived neurotrophic factor family receptors in the adult rat brain [J].