Expression of the Vanin Gene Family in Normal and Inflamed Human Skin: Induction by Proinflammatory Cytokines

被引：69

作者：

Jansen, Patrick A. M. ^{[1
,2
]}

Kamsteeg, Marijke ^{[1
,2
]}

Rodijk-Olthuis, Diana ^{[1
,2
]}

van Vlijmen-Willems, Ivonne M. J. J. ^{[1
,2
]}

de Jongh, Gys J. ^{[1
,2
]}

Bergers, Mieke ^{[1
,2
]}

Tjabringa, Geuranne S. ^{[1
,2
]}

Zeeuwen, Patrick L. J. M. ^{[1
,2
]}

Schalkwijk, Joost ^{[1
,2
]}

机构：

[1] Radboud Univ Nijmegen, Med Ctr, Dept Dermatol, NL-6525 ED Nijmegen, Netherlands

[2] Radboud Univ Nijmegen, Med Ctr, Nijmegen Ctr Mol Life Sci, NL-6525 ED Nijmegen, Netherlands

来源：

JOURNAL OF INVESTIGATIVE DERMATOLOGY | 2009年 / 129卷 / 09期

关键词：

ACTIVATED-RECEPTOR-GAMMA; HUMAN EPIDERMAL-KERATINOCYTES; ATOPIC-DERMATITIS; TRANSGLUTAMINASE ACTIVITY; PSORIATIC SKIN; DIFFERENTIATION; PANTETHEINASE; DISEASE; MOUSE; LOCALIZATION;

D O I：

10.1038/jid.2009.67

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

The vanin gene family encodes secreted and membrane-bound ectoenzymes that convert pantetheine into pantothenic acid and cysteamine. Recent studies in a mouse colitis model indicated that vanin-1 has proinflammatory activity and suggest that pantetheinases are potential therapeutic targets in inflammatory diseases. In a microarray analysis of epidermal gene expression of psoriasis and atopic dermatitis lesions, we identified vanin-3 as the gene showing the highest differential expression of all annotated genes that we studied (19-fold upregulation in psoriasis). Quantitative real-time PCR analysis confirmed the microarray data on vanin-3 and showed similar induction of vanin-1, but not of vanin-2, in psoriatic epidermis. Immunohistochemistry showed that vanin-3 is expressed in the differentiated epidermal layers. Using submerged and organotypic keratinocyte cultures, we found that vanin-1 and vanin-3 are induced at the mRNA and protein level by psoriasis-associated proinflammatory cytokines (Th17/Th1) but not by Th2 cytokines. We hypothesize that increased levels of pantetheinase activity are part of the inflammatory-regenerative epidermal differentiation program, and may contribute to the phenotype observed in psoriasis.

引用

页码：2167 / 2174

页数：8

共 51 条

[1] Vanin-1, a novel GPI-linked perivascular molecule involved in thymus homing [J].