Atomic force microscopy reveals binding of mRNA to microtubules mediated by two major mRNP proteins YB-1 and PABP

被引：34

作者：

Chernov, Konstantin G. ^{[1
,2
]}

Curmi, Patrick A. ^{[1
]}

Hamon, Loic ^{[1
]}

Mechulam, Alain ^{[1
]}

Ovchinnikov, Lev P. ^{[2
]}

Pastre, David ^{[1
]}

机构：

[1] UEVE, INSERM, Lab Struct Act Biomol Normales & Pathol, U829, F-91025 Evry, France

[2] Russian Acad Sci, Inst Prot Res, Pushchino 142290, Moscow Region, Russia

来源：

FEBS LETTERS | 2008年 / 582卷 / 19期

关键词：

microtubule; RNA; RNP; atomic force microscopy; YB-1; PABP;

D O I：

10.1016/j.febslet.2008.07.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A significant fraction of mRNAs is known to be associated in the form of mRNPs with microtubules for active transport. However, little is known about the interaction between mRNPs and microtubules and most of previous works were focused on molecular motor: microtubule interactions. Here, we have identified, via high resolution atomic force microscopy imaging, a significant binding of mRNA to microtubules mediated by two major mRNP proteins, YB-1 and PABP. This interaction with microtubules could be of critical importance for active mRNP traffic and for mRNP granule formation. A similar role may be fulfilled by other cationic mRNA partners. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：2875 / 2881

页数：7

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