New vectors for peptide display on the surface of filamentous bacteriophage

被引：28

作者：

Malik, P ^{[1
]}

Perham, RN ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT BIOCHEM,CAMBRIDGE CTR MOLEC RECOGNIT,CAMBRIDGE CB2 1QW,ENGLAND

来源：

GENE | 1996年 / 171卷 / 01期

基金：

英国惠康基金;

关键词：

hybrid phage; plasmid; epitope display; peptide libraries;

D O I：

10.1016/0378-1119(96)00070-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have modified the genome of the filamentous bacteriophage fd and also constructed a number of new vectors for the purpose of displaying peptides on the surface of the virion. These vectors facilitate the directional cloning of DNA encoding a peptide of interest at or near the N terminus of the major coat protein, the product of the bacteriophage gene VIII, and the construction of hybrid capsids in which the modified coat protein is interspersed with wild-type coat protein subunits.

引用

页码：49 / 51

页数：3

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