Molecular epidemiology of human immunodeficiency virus type 1 transmission in a heterosexual cohort of discordant couples in Zambia

被引:130
作者
Trask, SA
Derdeyn, CA
Fideli, U
Chen, YL
Meleth, S
Kasolo, F
Musonda, R
Hunter, E
Gao, F
Allen, S
Hahn, BH
机构
[1] Univ Alabama Birmingham, Sch Med, Dept Med, Birmingham, AL 35294 USA
[2] Univ Alabama Birmingham, Sch Med, Dept Microbiol, Birmingham, AL 35294 USA
[3] Univ Alabama Birmingham, Sch Publ Hlth, Dept Epidemiol & Int Hlth, Birmingham, AL 35294 USA
[4] Univ Lusaka, Teaching Hosp, Dept Pathol & Microbiol, Lusaka, Zambia
[5] Trop Dis Res Ctr, Ndola, Zambia
关键词
D O I
10.1128/JVI.76.1.397-405.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Most human immunodeficiency virus type 1 (HIV-1) transmissions in sub-Saharan Africa are believed to occur between married adults who are discordant for their HIV-1 infection status; however, no studies to date have investigated the molecular epidemiology of such transmission events. Here we report the genetic characterization of HIV-1 strains from 149 transmission pairs that were identified prospectively in a cohort of discordant couples in Lusaka, Zambia. Subgenomic gag, gp120, gp41, and/or long terminal repeat regions were amplified by PCR analysis of uncultured blood samples from both partners and sequenced without interim cloning. Pairwise genetic distances were calculated for the regions analyzed and compared to those of subtype-specific reference sequences as well as local controls. Sequence relationships were also examined by phylogenetic tree analysis. By these approaches, epidemiological linkage was established for the majority of transmission pairs. Viruses from 129 of the 149 couples (87%) were very closely related and clustered together in phylogenetic trees in a statistically highly significant manner. In contrast, viruses from 20 of the 149 couples (13%) were only distantly related in two independent genomic regions, thus ruling out transmission between the two partners. The great majority (95%) of transmitted viruses were of subtype C origin, although representatives of subtypes A, D, G, and J were also identified. There was no evidence for extensive transmission networks within the cohort, although two phylogenetic subclusters of viruses infecting two couples each were identified. Taken together, these data indicate that molecular epidemiological analyses of presumed transmission pairs are both feasible and required to determine behavioral, virological, and immunological correlates of heterosexual transmission in sub-Saharan Africa with a high level of accuracy.
引用
收藏
页码:397 / 405
页数:9
相关论文
共 45 条
  • [21] Method used to identify previously undiagnosed infections in the HIV outbreak at Glenochil prison
    Hutchinson, SJ
    Gore, SM
    Goldberg, DJ
    Yirrell, DL
    McGregor, J
    Bird, AG
    Leigh-Brown, AJ
    [J]. EPIDEMIOLOGY AND INFECTION, 1999, 123 (02) : 271 - 275
  • [23] Virtually full-length sequences of HIV type 1 subtype J reference strains
    Laukkanen, T
    Albert, J
    Liitsola, K
    Green, SD
    Carr, JK
    Leitner, T
    McCutchan, FE
    Salminen, MO
    [J]. AIDS RESEARCH AND HUMAN RETROVIRUSES, 1999, 15 (03) : 293 - 297
  • [24] Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination
    Lole, KS
    Bollinger, RC
    Paranjape, RS
    Gadkari, D
    Kulkarni, SS
    Novak, NG
    Ingersoll, R
    Sheppard, HW
    Ray, SC
    [J]. JOURNAL OF VIROLOGY, 1999, 73 (01) : 152 - 160
  • [25] McKenna SL, 1997, AIDS, V11, pS103
  • [26] Molecular cloning and phylogenetic analysis of human immunodeficiency virus type 1 subtype C: a set of 23 full-length clones from Botswana
    Novitsky, VA
    Montano, MA
    McLane, MF
    Renjifo, B
    Vannberg, F
    Foley, BT
    Ndung'u, TP
    Rahman, M
    Makhema, MJ
    Marlink, R
    Essex, M
    [J]. JOURNAL OF VIROLOGY, 1999, 73 (05) : 4427 - 4432
  • [27] Full-length genomic sequence of an HIV type 1 subtype G from Kinshasa
    Oelrichs, RB
    Vandamme, AM
    Van Laethem, K
    Debyser, Z
    McCutchan, FE
    Deacon, NJ
    [J]. AIDS RESEARCH AND HUMAN RETROVIRUSES, 1999, 15 (06) : 585 - 589
  • [28] NUCLEOTIDE-SEQUENCE OF A UGANDAN HIV-1 PROVIRUS REVEALS GENETIC DIVERSITY FROM OTHER HIV-1 ISOLATES
    ORAM, JD
    DOWNING, RG
    ROFF, M
    CLEGG, JCS
    SERWADDA, D
    CARSWELL, JW
    [J]. AIDS RESEARCH AND HUMAN RETROVIRUSES, 1990, 6 (09) : 1073 - 1078
  • [29] MOLECULAR EPIDEMIOLOGY OF HIV TRANSMISSION IN A DENTAL PRACTICE
    OU, CY
    CIESIELSKI, CA
    MYERS, G
    BANDEA, CI
    LUO, CC
    KORBER, BTM
    MULLINS, JI
    SCHOCHETMAN, G
    BERKELMAN, RL
    ECONOMOU, AN
    WITTE, JJ
    FURMAN, LJ
    SATTEN, GA
    MACINNES, KA
    CURRAN, JW
    JAFFE, HW
    [J]. SCIENCE, 1992, 256 (5060) : 1165 - 1171
  • [30] The global impact of HIV/AIDS
    Piot, P
    Bartos, M
    Ghys, PD
    Walker, N
    Schwartländer, B
    [J]. NATURE, 2001, 410 (6831) : 968 - 973