NMR-based metabonomic studies reveal changes in the biochemical profile of plasma and urine from pigs fed high-fibre rye bread

被引:52
作者
Bertram, HC
Knudsen, KEB
Serena, A
Malmendal, A
Nielsen, NC
Fretté, XC
Andersen, HJ
机构
[1] Danish Inst Agr Sci, Dept Food Sci, DK-8830 Tjele, Denmark
[2] Danish Inst Agr Sci, Dept Anim Hlth Welf & Nutr, DK-8830 Tjele, Denmark
[3] Univ Aarhus, Dept Chem, DK-8000 Aarhus C, Denmark
[4] Univ Aarhus, Interdisciplinary Nanosci Ctr iNANO, DK-8000 Aarhus C, Denmark
关键词
NMR; betaine; creatinine; wholegrain; metabolomics;
D O I
10.1079/BJN20061761
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
This study presents an NMR-based metabonomic approach to elucidate the overall endogenous biochemical effects of a wholegrain diet. Two diets with similar levels of dietary fibre and macronutrients, but with contrasting levels of wholegrain ingredients, were prepared from wholegrain rye (wholegrain diet (WGD)) and non-wholegrain wheat (non-wholegrain diet (NWD)) and fed to four pigs in a crossover design. Plasma samples were collected after 7 d on each diet, and H-1 NMR spectra were acquired on these. Partial least squares regression discriminant analysis (PLS-DA) on spectra obtained for plasma samples revealed that the spectral region at 3.25 parts per million dominates the differentiation between the two diets, as the WGD is associated with higher spectral intensity in this region. Spiking experiments and LC-MS analyses of the plasma verified that this spectral difference could be ascribed to a significantly higher content of betaine in WGD plasma samples compared with NWD samples. In an identical study with the same diets, urine samples were collected, and H-1 NMR spectra were acquired on these. PLS-DA on spectra obtained for urine samples revealed changes in the intensities of spectral regions, which could be ascribed to differences in the content of betaine and creatine/creatinine between the two diets, and LC-MS analyses verified a significantly lower content of creatinine in WGD urine samples compared with NWD urine samples. In conclusion, using an explorative approach, the present studies disclosed biochemical effects of a wholegrain diet on plasma betaine content and excretion of betaine and creatinine.
引用
收藏
页码:955 / 962
页数:8
相关论文
共 56 条
[41]   HIGH-RESOLUTION H-1-NMR STUDIES OF VERTEBRATE BLOOD AND PLASMA [J].
NICHOLSON, JK ;
BUCKINGHAM, MJ ;
SADLER, PJ .
BIOCHEMICAL JOURNAL, 1983, 211 (03) :605-615
[42]   Low dose betaine supplementation leads to immediate and long term lowering of plasma homocysteine in healthy men and women [J].
Olthof, MR ;
van Vliet, T ;
Boelsma, E ;
Verhoef, P .
JOURNAL OF NUTRITION, 2003, 133 (12) :4135-4138
[43]   Carbon tetrachloride-induced nephrotoxicity and protective effect of betaine in Sprague-Dawley rats [J].
Ozturk, F ;
Ucar, M ;
Ozturk, IC ;
Vardi, N ;
Batcioglu, K .
UROLOGY, 2003, 62 (02) :353-356
[44]   Effect of whole grains on insulin sensitivity in overweight hyperinsulinemic adults [J].
Pereira, MA ;
Jacobs, DR ;
Pins, JJ ;
Raatz, SK ;
Gross, MD ;
Slavin, JL ;
Seaquist, ER .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 2002, 75 (05) :848-855
[45]   A novel method to analyze betaine in chicken liver: Effect of dietary betaine and choline supplementation on the hepatic betaine concentration in broiler chicks [J].
Saarinen, MT ;
Kettunen, H ;
Pulliainen, K ;
Peuranen, S ;
Tiihonen, K ;
Remus, J .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (02) :559-563
[46]   Betaine supplementation decreases plasma homocysteine concentrations but does not affect body weight, body composition, or resting energy expenditure in human subjects [J].
Schwab, U ;
Törrönen, A ;
Toppinen, L ;
Alfthan, G ;
Saarinen, M ;
Aro, A ;
Uusitupa, M .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 2002, 76 (05) :961-967
[47]   Pharmacokinetics of oral betaine in healthy subjects and patients with homocystinuria [J].
Schwahn, BC ;
Hafner, D ;
Hohlfeld, T ;
Balkenhol, N ;
Laryea, MD ;
Wendel, U .
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY, 2003, 55 (01) :6-13
[48]  
Slavin JL, 1999, AM J CLIN NUTR, V70, p459S, DOI 10.1093/ajcn/70.3.459s
[49]   The role of whole grains in disease prevention [J].
Slavin, JL ;
Jacobs, D ;
Marquart, L ;
Wiemer, K .
JOURNAL OF THE AMERICAN DIETETIC ASSOCIATION, 2001, 101 (07) :780-785
[50]   Betaine analogues alter homocysteine metabolism in rats [J].
Slow, S ;
Lever, M ;
Lee, MB ;
George, PM ;
Chambers, ST .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2004, 36 (05) :870-880