Characterization of an alternatively spliced isoform of rat vesicle associated membrane protein-2 (VAMP-2)

被引：7

作者：

Mandic, R

Lowe, AW

机构：

[1] Stanford Univ, Dept Med, Stanford, CA 94305 USA

[2] Stanford Univ, Ctr Digest Dis, Stanford, CA 94305 USA

来源：

FEBS LETTERS | 1999年 / 451卷 / 02期

关键词：

membrane protein; secretory pathway; RNA splicing; secretion;

D O I：

10.1016/S0014-5793(99)00551-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

VAMPs are vesicle associated membrane proteins that are essential for secretion. A spliced isoform of rat VAMP-2, called VAMP-2B, is characterized in this study, The VAMP-2B transcript is the result of alternative RNA splicing in which an intron is retained. The predicted amino acid sequence of VAMP-2B differs from VAMP-2 at its carboxy-terminal end. Because recent studies have shown that VAMP's carboxy-terminal end influences the protein's sorting, the location of myc-epitope tagged VAMP-2B in PC12 cells was determined. Subcellular fractionation showed colocalization of myc-VAMP-2B and endogenous VAMP-2, Thus alternative RNA splicing does not affect VAMP-2 sorting in PC12 cells. (C) 1999 Federation of European Biochemical Societies.

引用

收藏

页码：209 / 213

页数：5

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