Forcing Stem Cells to Behave: A Biophysical Perspective of the Cellular Microenvironment

被引:272
作者
Sun, Yubing [1 ,2 ]
Chen, Christopher S. [4 ]
Fu, Jianping [1 ,2 ,3 ]
机构
[1] Univ Michigan, Integrated Biosyst & Biomech Lab, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[4] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA
来源
ANNUAL REVIEW OF BIOPHYSICS, VOL 41 | 2012年 / 41卷
基金
美国国家科学基金会;
关键词
stem cell fate; mechanotransduction; cellular microenvironment; mechanical forces; microtechnology/nanotechnology; HUMAN EMBRYONIC STEM; FOCAL ADHESION KINASE; VASCULAR SMOOTH-MUSCLE; SELF-RENEWAL; TGF-BETA; IN-VITRO; EXTRACELLULAR-MATRIX; CYTOSKELETAL DYNAMICS; MECHANICAL STRAIN; RHO-GTPASES;
D O I
10.1146/annurev-biophys-042910-155306
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Physical factors in the local cellular microenvironment, including cell shape and geometry, matrix mechanics, external mechanical forces, and nanotopographical features of the extracellular matrix, can all have strong influences on regulating stem cell fate. Stem cells sense and respond to these insoluble biophysical signals through integrin-mediated adhesions and the force balance between intracellular cytoskeletal contractility and the resistant forces originated from the extracellular matrix. Importantly, these mechanotransduction processes can couple with many other potent growth-factor-mediated signaling pathways to regulate stem cell fate. Different bioengineering tools and microscale/nanoscale devices have been successfully developed to engineer the physical aspects of the cellular microenvironment for stem cells, and these tools and devices have proven extremely powerful for identifying the extrinsic physical factors and their downstream intracellular signaling pathways that control stem cell functions.
引用
收藏
页码:519 / 542
页数:24
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