Simultaneous determination of five oxidative DNA lesions in human urine

被引：101

作者：

Ravanat, JL ^{[1
]}

Guicherd, P ^{[1
]}

Tuce, Z ^{[1
]}

Cadet, J ^{[1
]}

机构：

[1] CEA Grenoble, Dept Rech Fondamentale Mat Condensee, Serv Chim Inorgan & Biol, Lab Les Acides Nucleiques, F-38054 Grenoble 9, France

来源：

CHEMICAL RESEARCH IN TOXICOLOGY | 1999年 / 12卷 / 09期

关键词：

D O I：

10.1021/tx980194k

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A method, involving a HPLC prepurification followed by a GC/MS analysis, has been set up for the measurement of nucleic acid oxidation products in human urine. For this purpose, isotopically labeled internal standards have been prepared and used for isotope dilution mass spectrometric detection. Using this approach, four oxidized DNA bases, i.e., 5-hydroxyuracil, 5-(hydroxymethyl)uracil, 8-oxo-7,8-dihydroadenine, and 8-oxo-7,8-dihydroguanine, together with 8-oxo-7,8-dihydro-2'-deoxyguanosine have been simultaneously quantified in human urine samples. The levels of the oxidized nucleic acid constituents, as expressed in picomoles per milliliter, were determined to be, in decreasing order: 8-oxo-7,8-dihydroguanine (583 +/- 376) >5-(hydroxymethyl)uracil (121 +/- 56) > 5-hydroxyuracil (58 +/- 23) > 8-oxo-7,8-dihydro-2'-deoxyguanosine (30 +/- 15), 8-oxo-7,8-dihydroadenine (7 +/- 4). Attempts to determine the amount of 5,6-dihydroxy-5,6-dihydrothymine, 5-hydroxycytosine, and 2,6-diamino-4-hydroxy5-formamidopyrimidine using the above HPLC-GC/MS method were unsuccessful.

引用

页码：802 / 808

页数：7

共 32 条

[21] SYNTHESIS OF THE DIASTEREOMERS OF THYMIDINE GLYCOL, DETERMINATION OF CONCENTRATIONS AND RATES OF INTERCONVERSION OF THEIR CIS-TRANS EPIMERS AT EQUILIBRIUM AND DEMONSTRATION OF DIFFERENTIAL ALKALI LABILITY WITHIN DNA [J].