Construction and characterization of a series of vectors for Schizosaccharomyces pombe

被引：11

作者：

Adams, C ^{[1
]}

Haidar, D ^{[1
]}

Kamakaka, RT ^{[1
]}

机构：

[1] NICHHD, Unit Chromatin & Transcript, NIH, Bethesda, MD 20892 USA

来源：

YEAST | 2005年 / 22卷 / 16期

关键词：

Schizosaccharomyces pombe; vectors; genomic library;

D O I：

10.1002/yea.1332

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A set of vectors was created to allow cloning and expression studies in Schizosaccharomyces pombe. These vectors had a uniform backbone with an efficient Sz. pombe ARS, ARS3002, but different selectable markers - his3(+), leu1(+), ade6(+) and ura4(+). The vectors functioned efficiently as autonomously replicating plasmids that could also be converted into integrating vectors. The ura4(+)-containing vector was used to construct a Sz. pombe genomic library. Copyright (c) 2005 John Wiley & Sons, Ltd.

引用

页码：1307 / 1314

页数：8

共 13 条

[1]

3 ARS ELEMENTS CONTRIBUTE TO THE URA4-REPLICATION ORIGIN REGION IN THE FISSION YEAST, SCHIZOSACCHAROMYCES-POMBE [J].

DUBEY, DD ;

ZHU, JG ;

CARLSON, DL ;

SHARMA, K ;

HUBERMAN, JA .

EMBO JOURNAL, 1994, 13 (15) :3638-3647

[2]

The Schizosaccharomyces pombe hst4+ gene is a SIR2 homologue with silencing and centromeric functions [J].

Freeman-Cook, LL ;

Sherman, JM ;

Brachmann, CB ;

Allshire, RC ;

Boeke, JD ;

Pillus, L .

MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (10) :3171-3186

[3]

A 10-MINUTE DNA PREPARATION FROM YEAST EFFICIENTLY RELEASES AUTONOMOUS PLASMIDS FOR TRANSFORMATION OF ESCHERICHIA-COLI [J].

HOFFMAN, CS ;

WINSTON, F .

GENE, 1987, 57 (2-3) :267-272

[4]

KEENEY JB, 1994, GENETICS, V136, P849

[5]

Influence of a replication enhancer on the hierarchy of origin efficiencies within a cluster of DNA replication origins [J].

Kim, SM ;

Huberman, JA .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 288 (05) :867-882

[6]

Kim Soo-Mi, 2001, BMC Molecular Biology, V2, P1, DOI 10.1186/1471-2199-2-1

[7]

MAUNDRELL K, 1990, J BIOL CHEM, V265, P10857

[8]

TELOMERE-ASSOCIATED CHROMOSOME BREAKAGE IN FISSION YEAST RESULTS IN VARIEGATED EXPRESSION OF ADJACENT GENES [J].

NIMMO, ER ;

CRANSTON, G ;

ALLSHIRE, RC .

EMBO JOURNAL, 1994, 13 (16) :3801-3811

[9]

Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe [J].

Ohi, R ;

Feoktistova, A ;

Gould, KL .

GENE, 1996, 174 (02) :315-318

[10]

Choosing and using Schizosaccharomyces pombe plasmids [J].

Siam, R ;

Dolan, WP ;

Forsburg, SL .

METHODS, 2004, 33 (03) :189-198

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