Choosing and using Schizosaccharomyces pombe plasmids

被引：53

作者：

Siam, R

Dolan, WP

Forsburg, SL

机构：

[1] Salk Inst Biol Studies, Mol & Cell Biol Lab, La Jolla, CA 92037 USA

[2] Univ Calif San Diego, Div Biol Sci, La Jolla, CA 93093 USA

来源：

METHODS | 2004年 / 33卷 / 03期

关键词：

plasmid; promoter; marker; nmt; pombe; vector; episome; origin; selection; shut-off;

D O I：

10.1016/j.ymeth.2003.11.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A wide range of plasmids has been developed for molecular studies in the fission yeast Schizosaccharomyces pombe. This includes general purpose episomes, expression vectors, epitope tagging plasmids, and integration vectors. This review describes the typical features of S. pombe vectors, including replication origins, positive and negative selection markers, and constitutive and inducible promoter systems. We will also discuss vectors with epitope tags and how these can be used to modify episomal or endogenous gene sequences. Considerations for choosing and using a plasmid are presented and specialized methods are described. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：189 / 198

页数：10

共 55 条

[1] CLONING AND MANIPULATION OF THE SCHIZOSACCHAROMYCES-POMBE HIS7+ GENE AS A NEW SELECTABLE MARKER FOR MOLECULAR-GENETIC STUDIES [J].