Chronic marginal vitamin A status affects the distribution and function of T cells and natural T cells in aging Lewis rats

被引:20
作者
Dawson, HD
Ross, AC
机构
[1] Penn State Univ, Dept Nutr, University Pk, PA 16802 USA
[2] Penn State Univ, Grad Program Nutr, University Pk, PA 16802 USA
关键词
T-cells; natural T-cell; immunocompetence; vitamin A supplementation; rats;
D O I
10.1093/jn/129.10.1782
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Although both vitamin A (VA) deficiency and aging are independently associated with alterations in immune function, the effects of marginal VA status or VA supplementation on the immune system during aging were not studied. A long-term dietary study was conducted in a rat model of aging to quantify changes in T-cell populations in blood and spleen, including T-cells bearing a marker of natural killer (NKT) cells. The study included nine treatment groups [three levels of dietary VA: marginal (0.35 RE/kg diet), control (4.0 RE/kg diet), and supplemented (50 RE/kg diet); and three age groups: young (2-3 mo), middle-aged (8-10 mo), and old 20-22 mo); diets were fed continuously from weaning to the end of the study period. CD3(+)/CD4(+) T-cells decreased in percentage and number in blood with age, CD8(+) cells increased (%), and the CD4/CD8 ratio decreased. Conversely, aging was associated with increased NKT cells (phenotype CD3(intermediate)/NKR-P1(+)). Based on regression analysis of flow cytometry data, the phenotype of most NKT cells was CD3(intermediate)/NKR-P1(+)/CD28(-). NKT cells, which are most likely of extrathymic origin, accounted for most of the decrease in the CD4/CD8 ratio. Marginal VA status, particularly in older rats, was associated with increases in the percentage of CD8(+) T cells, percentage and number of NKT cells, and peripheral blood cell anti-CD3 epsilon-stimulated proliferative response, and decreases in the CD4/CD8 T-cell ratio and splenic cell interleukin-2 production. These differences and the reciprocal changes observed for NKT cells vs, T- and classical NK cells in aging VA-marginal rats suggest that low VA status during aging may increase the risk of infectious or neoplastic diseases that require a normal balance of T-cell or NK-cell responses.
引用
收藏
页码:1782 / 1790
页数:9
相关论文
共 40 条
[11]  
FORNI G, 1986, J NATL CANCER I, V76, P527
[12]   The effect of zinc and vitamin A supplementation on immune response in an older population [J].
Fortes, C ;
Forastiere, F ;
Agabiti, N ;
Fano, V ;
Pacifici, R ;
Virgili, F ;
Piras, G ;
Guidi, L ;
Bartoloni, C ;
Tricerri, A ;
Zuccaro, P ;
Ebrahim, S ;
Perucci, CA .
JOURNAL OF THE AMERICAN GERIATRICS SOCIETY, 1998, 46 (01) :19-26
[13]  
Franceschi C, 1995, Int Rev Immunol, V12, P57, DOI 10.3109/08830189509056702
[14]   PHENOTYPIC AND FUNCTIONAL-CHARACTERISTICS OF ACTIVATED CD8+ CELLS - A CD11B-CD28- SUBSET MEDIATES NONCYTOLYTIC FUNCTIONAL SUPPRESSION [J].
FREEDMAN, MS ;
RUIJS, TCG ;
BLAIN, M ;
ANTEL, JP .
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1991, 60 (02) :254-267
[15]   The age-associated decline in immune function of healthy individuals is not related to changes in plasma concentrations of beta-carotene, retinol, alpha-tocopherol or zinc [J].
Gardner, EM ;
Bernstein, ED ;
Dorfman, M ;
Abrutyn, E ;
Murasko, DM .
MECHANISMS OF AGEING AND DEVELOPMENT, 1997, 94 (1-3) :55-69
[16]  
GOODWIN JS, 1988, J GERONTOL, V43, P46
[17]   DEVELOPMENT OF AUTOREACTIVITY AND CHANGES OF T-CELL REPERTOIRE IN DIFFERENT STRAINS OF AGING MICE [J].
HOSONO, M ;
TOICHI, E ;
HOSOKAWA, M ;
IMAMURA, S ;
GYOTOKU, J ;
KATSURA, Y ;
HOSOKAWA, T .
MECHANISMS OF AGEING AND DEVELOPMENT, 1995, 78 (03) :197-214
[18]   Induction of MHC Class I restricted human suppressor T cells by peptide priming in vitro [J].
Jiang, SP ;
Tugulea, S ;
Pennesi, G ;
Liu, ZR ;
Mulder, A ;
Lederman, S ;
Harris, P ;
Cortesini, R ;
Suciu-Foca, N .
HUMAN IMMUNOLOGY, 1998, 59 (11) :690-699
[19]  
Kidd P, 1997, MANUAL CLIN LAB IMMU, P229
[20]   Cloning, functional activities and in vivo tissue distribution of rat NKR-P1(+) TCR alpha beta(+) cells [J].
Knudsen, E ;
Seierstad, T ;
Vaage, JT ;
Naper, C ;
Benestad, HB ;
Rolstad, B ;
Maghazachi, AA .
INTERNATIONAL IMMUNOLOGY, 1997, 9 (07) :1043-1051