Improved high-performance liquid chromatography of amino acids derivatised with 9-fluorenylmethyl chloroformate

被引：59

作者：

Ou, KL

Wilkins, MR

Yan, JX

Gooley, AA

Fung, Y

Sheumack, D

Williams, KL

机构：

[1] MACQUARIE UNIV,CTR ANALYT BIOTECHNOL,SYDNEY,NSW 2109,AUSTRALIA

[2] GBC SCI EQUIPMENT,DANDENONG,VIC 3175,AUSTRALIA

[3] HANITRO PTY LTD,ANALYT SCI CONSULTANTS,S MATOATA,NSW 2756,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 723卷 / 02期

基金：

澳大利亚研究理事会;

关键词：

derivatization; LC; amino acids;

D O I：

10.1016/0021-9673(95)00879-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved high-performance liquid chromatography method for the separation of amino acids derivatised with 9-fluorenylmethyl chloroformate (Fmoc) is described. This method, in conjunction with a multi-tasking program, not only allows high automatic throughput but also offers baseline resolution of the common Fmoc-amino acids from a linear acetonitrile gradient, greater chromatographic reproducibility over the life of the column (800 runs) and easy column regeneration. The methods described are shown to be suitable for analysis of hydrolysates of proteins from two-dimensional gels for protein identification purposes, and will also be useful for routine quality control and screening of biological samples.

引用

页码：219 / 225

页数：7

共 17 条

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