Fractionated marine invertebrate extract libraries for drug discovery

被引：39

作者：

Bugni, Tim S. ^{[1
]}

Harper, Mary Kay ^{[1
]}

McCulloch, Malcolm W. B. ^{[1
]}

Reppart, Jason ^{[1
]}

Ireland, Chris M. ^{[1
]}

机构：

[1] Univ Utah, Dept Med Chem, Salt Lake City, UT 84112 USA

来源：

MOLECULES | 2008年 / 13卷 / 06期

关键词：

sponge; tunicate; prefractionation; HP20SS; marine natural products; LCMS;

D O I：

10.3390/molecules13061372

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The high-throughput screening and drug discovery paradigm has necessitated a change in preparation of natural product samples for screening programs. In an attempt to improve the quality of marine natural products samples for screening, several fractionation strategies were investigated. The final method used HP20SS as a solid support to effectively desalt extracts and fractionate the organic components. Additionally, methods to integrate an automated LCMS fractionation approach to shorten discovery time lines have been implemented.

引用

页码：1372 / 1383

页数：12

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