A Simple Polymerase Chain Reaction-Based Method for the Construction of Recombinase-Mediated Cassette Exchange Donor Vectors

被引：22

作者：

Bateman, Jack R. ^{[1
]}

Wu, C. -ting ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

来源：

GENETICS | 2008年 / 180卷 / 03期

关键词：

D O I：

10.1534/genetics.108.094508

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the Phi C31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.

引用

页码：1763 / 1766

页数：4

共 13 条

[1] Site-specific transformation of Drosophila via φC31 integrase-mediated cassette exchange [J].

Bateman, Jack R. ;

Lee, Anne M. ;

Wu, C. -ting .

GENETICS, 2006, 173 (02) :769-777

[2] An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases [J].

Bischof, Johannes ;

Maeda, Robert K. ;

Hediger, Monika ;

Karch, Francois ;

Basler, Konrad .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (09) :3312-3317

[3] Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice [J].

Branda, CS ;

Dymecki, SM .

DEVELOPMENTAL CELL, 2004, 6 (01) :7-28

[4] Site-specific chromosomal integration in mammalian cells: Highly efficient CRE recombinase-mediated cassette exchange [J].

Feng, YQ ;

Seibler, J ;

Alami, R ;

Eisen, A ;

Westerman, KA ;

Leboulch, P ;

Fiering, S ;

Bouhassira, EE .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 292 (04) :779-785

[5] Construction of transgenic Drosophila by using the site-specific integrase from phage φC31 [J].

Groth, AC ;

Fish, M ;

Nusse, R ;

Calos, MP .

GENETICS, 2004, 166 (04) :1775-1782

[6] A phage integrase directs efficient site-specific integration in human cells [J].

Groth, AC ;

Olivares, EC ;

Thyagarajan, B ;

Calos, MP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (11) :5995-6000

[7]

HORTON RM, 1990, BIOTECHNIQUES, V8, P528

[8] Road to precision: recombinase-based targeting technologies for genome engineering [J].

Nov, Dagmar ;

Gama-Norton, Leonor ;

Riemer, Pamela ;

Sandhu, Upneet ;

Schucht, Roland ;

Hauser, Hansjorg .

CURRENT OPINION IN BIOTECHNOLOGY, 2007, 18 (05) :411-419

[9] DNA cassette exchange in ES cells mediated by FLP recombinase:: An efficient strategy for repeated modification of tagged loci by marker-free constructs [J].

Seibler, J ;

Schübeler, D ;

Fiering, S ;

Groudine, M ;

Bode, J .

BIOCHEMISTRY, 1998, 37 (18) :6229-6234

[10] TRANSPOSITION OF CLONED P ELEMENTS INTO DROSOPHILA GERM LINE CHROMOSOMES [J].

SPRADLING, AC ;

RUBIN, GM .

SCIENCE, 1982, 218 (4570) :341-347

← 1 2 →