Simultaneous detection of near-field topographic and fluorescence images of human chromosomes via scanning near-field optical/atomic-force microscopy (SNOAM)

被引：25

作者：

Iwabuchi, S

Muramatsu, H

Chiba, N

Kinjo, Y

Murakami, Y

Sakaguchi, T

Yokoyama, K

Tamiya, E

机构：

[1] JAPAN ADV INST SCI & TECHNOL,SCH MAT SCI,TATSUNOKUCHI,ISHIKAWA 92312,JAPAN

[2] SEIKO INSTRUMENTS INC,RES LAB ADV TECHNOL,MATSUDO,CHIBA 271,JAPAN

[3] TOKYO METROPOLITAN ISOTOPE RES INST,DEPT RADIAT RES,SETAGAYA KU,TOKYO 158,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 08期

关键词：

D O I：

10.1093/nar/25.8.1662

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Scanning near-field optical/atomic-force microscopy (SNOAM) provided us with simultaneous topographical ansi optical images of human chromosomes using a sharp and bent optical fiber as a near-field optical probe. Native chromosomes were spread out onto a coverslip easing the surface-spreading whole-mount method. The SNOAM system does not need pretreatment of samples such as metal coating or chemical immobilization. Near-field topographic and fluorescence images provided useful information on native chromosome structure.

引用

页码：1662 / 1663

页数：2

共 9 条

[1] SINGLE MOLECULES OBSERVED BY NEAR-FIELD SCANNING OPTICAL MICROSCOPY [J].