Epigallocatechin-3-gallate inhibits basic fibroblast growth factor - Induced intracellular signaling transduction pathway in rat aortic smooth muscle cells

被引:35
作者
Hwang, KC
Lee, KH
Jang, YS
Yun, YP
Chung, KH [1 ]
机构
[1] Yonsei Univ, Coll Med, Cardiovasc Res Inst, Seoul 120752, South Korea
[2] Yonsei Univ, Coll Med, Brain Korea Project Med Sci 21, Seoul 120752, South Korea
[3] Yonsei Univ, Coll Med, Dept Internal Med, Div Cardiol, Seoul 120752, South Korea
[4] Chungbuk Natl Univ, Coll Pharm, Cheongju, South Korea
关键词
anti-proliferation; bFGF stimulation; c-jun expression; epigallocathechin-3-gallate; smooth muscle cell;
D O I
10.1097/00005344-200202000-00014
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Daily green tea drinking showed preventive effects on the progression of atherosclerosis. Although epigallocatechin-3-gallate [EGCG] has anti-proliferative effects on various cells, relatively little is known about the molecular mechanisms of the anti-proliferative effects of EGCG. To determine whether the transduction signals and protooncogene expression were affected by EGCG, this study investigated the molecular mechanism of the anti-proliferative effects in basic fibroblast growth factor (bFGF)-stimulated rat aortic smooth muscle cells (RAoSMCs). EGCG inhibited the proliferative response stimulated by 10% fetal bovine serum dose dependently in RAoSMCs (median inhibitory concentration [IC50]: 28.4 x 10(-6) M). EGCG also inhibited the migration of bFGF-stimulated RAoSMCs in a dose-dependent manner, showing that 21.8 x 10(-6) M of EGCG significantly inhibited the migration by 75+/-5% in comparison with bFGF-stimulated migration. In RAoSMCs, EGCG dramatically inhibited Ras activation and c-jun N-terminal kinase (JNK) activity without affecting protein kinase C expression. Induction of c-jun mRNA stimulated by bFGF was significantly reduced dose dependently up to 87.3 x 10(-6) M of EGCG. These results indicate that the anti-proliferative effect of EGCG on RAoSMCs is partly Ras/JNK mediated, independent of protein kinase C, and is attributable to the downregulation of c-jun expression.
引用
收藏
页码:271 / 277
页数:7
相关论文
共 27 条
[1]   Epigallocathechin-3 gallate selectively inhibits the PDGF-BB-induced intracellular signaling transduction pathway in vascular smooth muscle cells and inhibits transformation of sis-transfected NIH 3T3 fibroblasts and human glioblastoma cells (A172) [J].
Ahn, HY ;
Hadizadeh, KR ;
Seul, C ;
Yun, YP ;
Vetter, H ;
Sachinidis, A .
MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (04) :1093-1104
[2]   Biological roles of fibroblast growth factor-2 [J].
Bikfalvi, A ;
Klein, S ;
Pintucci, G ;
Rifkin, DB .
ENDOCRINE REVIEWS, 1997, 18 (01) :26-45
[3]   Angiogenesis inhibited by drinking tea [J].
Cao, YH ;
Cao, RH .
NATURE, 1999, 398 (6726) :381-381
[4]   SMOOTH-MUSCLE CELL IN CULTURE [J].
CHAMLEYCAMPBELL, J ;
CAMPBELL, GR ;
ROSS, R .
PHYSIOLOGICAL REVIEWS, 1979, 59 (01) :1-61
[5]   JNK1 - A PROTEIN-KINASE STIMULATED BY UV-LIGHT AND HA-RAS THAT BINDS AND PHOSPHORYLATES THE C-JUN ACTIVATION DOMAIN [J].
DERIJARD, B ;
HIBI, M ;
WU, IH ;
BARRETT, T ;
SU, B ;
DENG, TL ;
KARIN, M ;
DAVIS, RJ .
CELL, 1994, 76 (06) :1025-1037
[6]   Troglitazone inhibits angiotensin II-induced DNA synthesis and migration in vascular smooth muscle cells [J].
Graf, K ;
Xi, XP ;
Hsueh, WA ;
Law, RE .
FEBS LETTERS, 1997, 400 (01) :119-121
[7]   BACTERICIDAL CATECHINS DAMAGE THE LIPID BILAYER [J].
IKIGAI, H ;
NAKAE, T ;
HARA, Y ;
SHIMAMURA, T .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1147 (01) :132-136
[8]   Why drinking green tea could prevent cancer [J].
Jankun, J ;
Selman, SH ;
Swiercz, R ;
SkrzypczakJankun, E .
NATURE, 1997, 387 (6633) :561-561
[9]   JNK2 CONTAINS A SPECIFICITY-DETERMINING REGION RESPONSIBLE FOR EFFICIENT C-JUN BINDING AND PHOSPHORYLATION [J].
KALLUNKI, T ;
SU, B ;
TSIGELNY, I ;
SLUSS, HK ;
DERIJARD, B ;
MOORE, G ;
DAVIS, R ;
KARIN, M .
GENES & DEVELOPMENT, 1994, 8 (24) :2996-3007
[10]   Antithrombotic activities of green tea catechins and (-)-epigallocatechin gallate [J].
Kang, WS ;
Lim, IH ;
Yuk, DY ;
Chung, KH ;
Park, JB ;
Yoo, HS ;
Yun, YP .
THROMBOSIS RESEARCH, 1999, 96 (03) :229-237