Assembly of actin scaffolds at ponticulin-containing supported phospholipid bilayers

被引：12

作者：

Johnson, BRG

Bushby, RJ

Colyer, J

Evans, SD ^{[1
]}

机构：

[1] Univ Leeds, Sch Phys & Astron, Leeds LS2 9JT, W Yorkshire, England

[2] Univ Leeds, Ctr Sell Organising Mol Syst, Leeds LS2 9JT, W Yorkshire, England

来源：

BIOPHYSICAL JOURNAL | 2006年 / 90卷 / 03期

基金：

英国工程与自然科学研究理事会;

关键词：

D O I：

10.1529/biophysj.105.076521

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Phospholipid vesicles containing ponticulin have been used to form solid supported and tethered bilayer lipid membranes. The ponticulin serves as both a nucleation site for actin polymerization as well as a binding site for F-actin. Studies of F-actin binding to such bilayers have demonstrated the formation of an in vitro actin scaffold. The dissociation constant for the binding of F-actin. laments to a ponticulin-containing tethered bilayer was found to be 11 +/- 5 nM, indicative of high affinity binding.

引用

页码：L21 / L23

页数：3

共 17 条

[11] Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides [J].