Cross-protective effect of acid-adapted Salmonella enterica on resistance to lethal acid and cold stress conditions

被引：42

作者：

Xu, H. ^{[2
]}

Lee, H. Y. ^{[1
,2
]}

Ahn, J. ^{[1
,2
]}

机构：

[1] Kangwon Natl Univ, Inst Biosci & Biotechnol, Chunchon 200701, Gangwon, South Korea

[2] Kangwon Natl Univ, Div Biomat Engn, Chunchon 200701, Gangwon, South Korea

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2008年 / 47卷 / 04期

关键词：

acid injury; acid tolerance; Gompertz; Salmonella; viable but nonculturable; Weibull;

D O I：

10.1111/j.1472-765X.2008.02429.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: To evaluate the cross-protected Salmonella enterica cells under acid and cold stress conditions. Methods and Results: The acid-adapted S. enterica cells were exposed to pH 4.0 at 4 and 20 degrees C. Recovery of sublethally injured cells was estimated by the difference between the counts obtained on trypticase soy agar (TSA) and xylose lysine desoxycholate (XLD) agar. The survival curves of nonadapted and acid-adapted S. enterica cells at pH 4.0 were fitted with Weibull distribution model. The recovery behaviour of injured S. enterica cells was estimated by the modified Gompertz parameters. Acid-adapted S. enterica were more resistant to subsequent acid shock than the nonadapted cells. The numbers of nonadapted S. enterica cells were decreased by 4.57 and 7.55 log CFU ml(-1) at 4 and 20 degrees C after 12-day acid challenge, respectively. The acid adaptation induced cross-protection and viable nonculturable (VBNC) state against low acid and cold stresses. The 7-h adaptation showed the least recovery of injured cells. Conclusion: The results suggest that acid-adapted S. enterica cells induced acid tolerance response and VBNC state. Significance and Impact of the Study: These results provide useful information for understanding the induction of cross-protected and VBNC pathogens under various stresses, which might be needed in designing new food preservation strategies.

引用

页码：290 / 297

页数：8

共 44 条

[21]

Lázaro B, 1999, APPL ENVIRON MICROB, V65, P4677

[22] ACID ADAPTATION INDUCES CROSS-PROTECTION AGAINST ENVIRONMENTAL STRESSES IN SALMONELLA-TYPHIMURIUM [J].

LEYER, GJ ;

JOHNSON, EA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (06) :1842-1847

[23] ACID ADAPTATION PROMOTES SURVIVAL OF SALMONELLA SPP IN CHEESE [J].

LEYER, GJ ;

JOHNSON, EA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (06) :2075-2080

[24] Resuscitation of acid-injured Salmonella in enrichment broth, in apple juice and on the surfaces of fresh-cut cucumber and apple [J].

Liao, CH ;

Fett, WF .

LETTERS IN APPLIED MICROBIOLOGY, 2005, 41 (06) :487-492

[25] MEMBRANE FATTY-ACID AND VIRULENCE CHANGES IN THE VIABLE BUT NONCULTURABLE STATE OF VIBRIO-VULNIFICUS [J].

LINDER, K ;

OLIVER, JD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (11) :2837-2842

[26] USE OF A MODIFIED GOMPERTZ EQUATION TO MODEL NONLINEAR SURVIVAL CURVES FOR LISTERIA-MONOCYTOGENES SCOTT-A [J].

LINTON, RH ;

CARTER, WH ;

PIERSON, MD ;

HACKNEY, CR .

JOURNAL OF FOOD PROTECTION, 1995, 58 (09) :946-954

[27] Inhibition of the resuscitation from the viable but non-culturable state in Enterococcus faecalis [J].

Lleo, Maria del Mar ;

Benedetti, Dennis ;

Tafi, Maria Carla ;

Signoretto, Caterina ;

Canepari, Pietro .

ENVIRONMENTAL MICROBIOLOGY, 2007, 9 (09) :2313-2320

[28]

Lleò MD, 2000, APPL ENVIRON MICROB, V66, P4564

[29] Temperature and growth-phase effects on Aeromonas hydrophila survival in natural seawater microcosms:: role of protein synthesis and nucleic acid content on viable but temporarily nonculturable response [J].

Maalej, S ;

Denis, M ;

Dukan, S .

MICROBIOLOGY-SGM, 2004, 150 :181-187

[30] Induction of an adaptive tolerance response in the foodborne pathogen, Campylobacter jejuni [J].

Murphy, C ;

Carroll, C ;

Jordan, KN .

FEMS MICROBIOLOGY LETTERS, 2003, 223 (01) :89-93

← 1 2 3 4 5 →