Mapping Protein-Protein Proximity in the Purinosome

被引：55

作者：

Deng, Yijun ^{[1
]}

Gam, Jongsik ^{[2
]}

French, Jarrod B. ^{[1
]}

Zhao, Hong ^{[1
]}

An, Songon ^{[3
]}

Benkovic, Stephen J. ^{[1
]}

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

[2] Korea Res Inst Biosci & Biotechnol, Taejon 306809, South Korea

[3] Univ Maryland, Dept Chem & Biochem, Baltimore, MD 21250 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2012年 / 287卷 / 43期

基金：

美国国家卫生研究院;

关键词：

MULTIENZYME COMPLEX; PURINE BIOSYNTHESIS; PURIFICATION; PATHWAY; CELLS;

D O I：

10.1074/jbc.M112.407056

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzymes in the human de novo purine synthesis pathway were found to form a cellular complex, the purinosome, upon culturing cells in purine-depleted medium (An, S., Kumar R., Sheets, E. D., and Benkovic, S. J. (2008) Science 320, 103-106). Purinosome formation and dissociation were found to be modulated by several factors, including the microtubule network and cell signaling involving protein phosphorylation. To determine whether the pathway enzymes are in physical contact, we probed for the protein-protein interactions (PPIs) within the purinosome with a novel application of the Tango PPI reporter system (Barnea, G., Strapps, W., Herrada, G., Berman, Y., Ong, J., Kloss, B., Axel, R., and Lee, K. J. (2008) Proc. Natl. Acad. Sci. U.S.A. 105, 64-69). We found PPIs among all six enzymes within the pathway and evidence for a core involving the first three enzymes. We also captured purinosomes under both purine-rich and purine-depleted conditions. The results provide additional insights into the transient nature and topography of the purinosome.

引用

页码：36201 / 36207

页数：7

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