Localization of the tandem pore domain K+ channel KCNK5 (TASK-2) in the rat central nervous system

被引:48
作者
Gabriel, A
Abdallah, M
Yost, CS
Winegar, BD
Kindler, CH [1 ]
机构
[1] Univ Basel, Kantonsspital, Dept Anesthesia, CH-4031 Basel, Switzerland
[2] Univ Cologne, Dept Anesthesia & Operat Intens Care Med, D-50924 Cologne, Germany
[3] Univ Calif San Francisco, Dept Anesthesia & Perioperat Care, San Francisco, CA 94143 USA
来源
MOLECULAR BRAIN RESEARCH | 2002年 / 98卷 / 1-2期
基金
美国国家卫生研究院;
关键词
potassium channel; 2P domain; baseline conductance; expression; central nervous system; volatile anesthetics; immunohistochemistry;
D O I
10.1016/S0169-328X(01)00330-8
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Tandem pore domain K+ channels (2P K+ channels) are responsible for background K+ currents. 2P K channels are the most numerous encoded K+ channels in the Caenorhabditis elegans and Drosophila melanogaster genomes and to date 14 human 2P K+ channels have been identified. The 2P K+ channel TASK-2 (also named KCNK5) is sensitive to changes in extracellular pH, inhibited by local anesthetics and activated by volatile anesthetics, While TASK-1 has been shown to be involved in controlling neuronal cell excitability, much less is known about the cellular expression and function of TASK-2, originally cloned from human kidney. Previous studies demonstrated TASK-2 mRNA expression in high abundance in human kidney, liver, and pancreas, but only low expression in mouse brain or even absent expression in human brain was reported. In this study we have used immunohistochemical methods to localize TASK-2 at the cellular level in the rat central nervous system. TASK-2 immunoreactivity is prominently found in the rat hippocampal formation with the strongest staining observed in the pyramidal cell layer and in the dentate gyrus, and the Purkinje and granule cells of cerebellum. Additional immunofluorescence studies in cultured cerebellar granule cells demonstrate TASK-2 localization to the neuronal soma and to the proximal regions of neurites of cerebellar granule cells. The superficial layers of spinal cord and small-diameter neurons of dorsal root ganglia also showed strong TASK-2 immunoreactivity. These results suggest a possible involvement of TASK-2 in central mechanisms for controlling cell excitability and in peripheral signal transduction. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:153 / 163
页数:11
相关论文
共 60 条
[1]   Neurobiology of the Caenorhabditis elegans genome [J].
Bargmann, CI .
SCIENCE, 1998, 282 (5396) :2028-2033
[2]   Adaptive regulation of neuronal excitability by a voltage-independent potassium conductance [J].
Brickley, SG ;
Revilla, V ;
Cull-Candy, SG ;
Wisden, W ;
Farrant, M .
NATURE, 2001, 409 (6816) :88-92
[3]   Neurobiology: The acid test for resting potassium channels [J].
Brown, DA .
CURRENT BIOLOGY, 2000, 10 (12) :R456-R459
[4]   An oxygen-, acid- and anaesthetic-sensitive TASK-like background potassium channel in rat arterial chemoreceptor cells [J].
Buckler, KJ ;
Williams, BA ;
Honore, E .
JOURNAL OF PHYSIOLOGY-LONDON, 2000, 525 (01) :135-142
[5]  
Chapman CG, 2000, MOL BRAIN RES, V82, P74
[6]   TWIK-2, a new weak inward rectifying member of the tandem pore domain potassium channel family [J].
Chavez, RA ;
Gray, AT ;
Zhao, BB ;
Kindler, CH ;
Mazurek, MJ ;
Mehta, Y ;
Forsayeth, JR ;
Yost, CS .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (12) :7887-7892
[7]   Expression of TWIK-1, a novel weakly inward rectifying potassium channel in rat kidney [J].
Cluzeaud, F ;
Reyes, R ;
Escoubet, B ;
Fay, M ;
Lazdunski, M ;
Bonvalet, JP ;
Lesage, F ;
Farman, N .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1998, 275 (06) :C1602-C1609
[8]   ANESTHETIC ACTIONS WITHIN THE SPINAL-CORD - CONTRIBUTIONS TO THE STATE OF GENERAL-ANESTHESIA [J].
COLLINS, JG ;
KENDIG, JJ ;
MASON, P .
TRENDS IN NEUROSCIENCES, 1995, 18 (12) :549-553
[9]   TASK (TWIK-related acid-sensitive K+ channel) is expressed in glomerulosa cells of rat adrenal cortex and inhibited by angiotensin II [J].
Czirják, G ;
Fischer, T ;
Spät, A ;
Lesage, F ;
Enyedi, P .
MOLECULAR ENDOCRINOLOGY, 2000, 14 (06) :863-874
[10]  
de Miera Eleazar Vega-Saenz, 2001, Journal of Neurophysiology (Bethesda), V86, P130