Characterization of a cartilage-derived 66-kDa protein (RGD-CAP/beta ig-h3) that binds to collagen

被引:144
作者
Hashimoto, K
Noshiro, M
Ohno, S
Kawamoto, T
Satakeda, H
Akagawa, Y
Nakashima, K
Okimura, A
Ishida, H
Okamoto, T
Pan, H
Shen, M
Yan, WQ
Kato, Y
机构
[1] HIROSHIMA UNIV,SCH DENT,DEPT BIOCHEM,MINAMI KU,HIROSHIMA 734,JAPAN
[2] HIROSHIMA UNIV,SCH DENT,DEPT REMOVAL PROSTHODONT,MINAMI KU,HIROSHIMA 734,JAPAN
[3] HIROSHIMA UNIV,SCH DENT,DEPT ORTHODONT,HIROSHIMA 734,JAPAN
[4] HIROSHIMA UNIV,SCH DENT,DEPT ORAL & MAXILLOFACIAL SURG 1,MINAMI KU,HIROSHIMA 734,JAPAN
[5] NIPPON HOECHST MARION ROUSSEL,LAB BONE RES,KAWAGOE,SAITAMA 350,JAPAN
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1997年 / 1355卷 / 03期
关键词
collagen-binding protein; cartilage; chondrocyte; collagen; RGD; TGF-beta;
D O I
10.1016/S0167-4889(96)00147-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A 66-kDa collagen fiber-associated protein (RGD-CAP) was isolated from a fiber-rich fraction of pig cartilage by ultrafiltration and collagen-affinity chromatography. Amino acid sequencing and cDNA cloning indicated that the RGD-CAP is identical or closely related to beta ig-h3 protein which is induced in human adenocarcinoma cells by transforming growth factor-beta (TGF-beta) (Skonier, J., Neubauer, M., Madisen, L., Bennett, K., Plowman, G.D., and Purchio, A.F. (1992) DNA Cell. Biol. 11, 511-522). The RGD-CAP, as well as beta ig-h3, has the RGD sequence in the C-terminal region. The native RGD-CAP bound to type I, II, and IV collagens even in the presence of 1 M NaCl. A recombinant preparation of RGD-CAP expressed in Escherichia coli cells also bound to collagen but not to gelatin. The RGD-CAP mRNA was expressed in chondrocytes throughout all stages, although the expression level was highest during the prehypertrophic stage. In addition, TGF-beta increased the RGD-CAP mRNA level in chondrocyte cultures. Since RGD-CAP transcripts were found in most tissues, this novel collagen-binding protein may play an important role in cell-collagen interactions in various tissues including developing cartilage.
引用
收藏
页码:303 / 314
页数:12
相关论文
共 39 条
  • [1] BROWN RA, 1990, RHEUMATOLOGY, V17, P65
  • [2] DROSOPHILA FASCICLIN-I IS A NOVEL HOMOPHILIC ADHESION MOLECULE THAT ALONG WITH FASCICLIN-III CAN MEDIATE CELL SORTING
    ELKINS, T
    HORTSCH, M
    BIEBER, AJ
    SNOW, PM
    GOODMAN, CS
    [J]. JOURNAL OF CELL BIOLOGY, 1990, 110 (05) : 1825 - 1832
  • [3] ERCOLANI L, 1988, J BIOL CHEM, V263, P15335
  • [4] CDNA FROM HUMAN OCULAR CILIARY EPITHELIUM HOMOLOGOUS TO BETA-IG-H3 IS PREFERENTIALLY EXPRESSED AS AN EXTRACELLULAR PROTEIN IN THE CORNEAL EPITHELIUM
    ESCRIBANO, J
    HERNANDO, N
    GHOSH, S
    CRABB, J
    COCAPRADOS, M
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 1994, 160 (03) : 511 - 521
  • [5] REDUCED AMOUNTS OF CARTILAGE COLLAGEN FIBRILS AND GROWTH PLATE ANOMALIES IN TRANSGENIC MICE HARBORING A GLYCINE-TO-CYSTEINE MUTATION IN THE MOUSE TYPE-II PROCOLLAGEN ALPHA-1-CHAIN GENE
    GAROFALO, S
    VUORIO, E
    METSARANTA, M
    ROSATI, R
    TOMAN, D
    VAUGHAN, J
    LOZANO, G
    MAYNE, R
    ELLARD, J
    HORTON, W
    DECROMBRUGGHE, B
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) : 9648 - 9652
  • [6] ANALYSIS OF ALPHA(1)BETA(1), ALPHA(2)BETA(1) AND ALPHA(3)BETA(1) INTEGRINS IN CELL-COLLAGEN INTERACTIONS - IDENTIFICATION OF CONFORMATION DEPENDENT ALPHA(1)BETA(1) BINDING-SITES IN COLLAGEN TYPE-I
    GULLBERG, D
    GEHLSEN, KR
    TURNER, DC
    AHLEN, K
    ZIJENAH, LS
    BARNES, MJ
    RUBIN, K
    [J]. EMBO JOURNAL, 1992, 11 (11) : 3865 - 3873
  • [7] HASCALL VC, 1974, J BIOL CHEM, V249, P4242
  • [8] IWAMOTO M, 1991, J BIOL CHEM, V266, P461
  • [9] CHARACTERIZATION OF THE MINERALIZATION PROCESS IN CULTURES OF RABBIT GROWTH PLATE CHONDROCYTES
    JIKKO, A
    AOBA, T
    MURAKAMI, H
    TAKANO, Y
    IWAMOTO, M
    KATO, Y
    [J]. DEVELOPMENTAL BIOLOGY, 1993, 156 (02) : 372 - 380
  • [10] TERMINAL DIFFERENTIATION AND CALCIFICATION IN RABBIT CHONDROCYTE CULTURES GROWN IN CENTRIFUGE TUBES - REGULATION BY TRANSFORMING GROWTH FACTOR-BETA AND SERUM FACTORS
    KATO, Y
    IWAMOTO, M
    KOIKE, T
    SUZUKI, F
    TAKANO, Y
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) : 9552 - 9556