A bacteriophage endolysin-based electrochemical impedance biosensor for the rapid detection of Listeria cells

被引：104

作者：

Tolba, Mona ^{[1
]}

Ahmed, Minhaz Uddin ^{[1
]}

Tlili, Chaker ^{[1
]}

Eichenseher, Fritz ^{[2
]}

Loessner, Martin J. ^{[2
]}

Zourob, Mohammed ^{[1
]}

机构：

[1] INRS EMT, Varennes, PQ J3X 1S2, Canada

[2] Swiss Fed Inst Technol, Inst Food Nutr & Hlth, CH-8092 Zurich, Switzerland

来源：

ANALYST | 2012年 / 137卷 / 24期

基金：

加拿大自然科学与工程研究理事会;

关键词：

ESCHERICHIA-COLI O157-H7; FLIGHT MASS-SPECTROMETRY; SELF-ASSEMBLED MONOLAYER; WALL-BINDING DOMAINS; IMPEDIMETRIC DETECTION; MONOCYTOGENES; IMMOBILIZATION; SALMONELLA; SEPARATION; SURFACE;

D O I：

10.1039/c2an35988j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The objective of this study was to develop a biosensor using the cell wall binding domain (CBD) of bacteriophage-encoded peptidoglycan hydrolases (endolysin) immobilized on a gold screen printed electrode (SPE) and subsequent electrochemical impedance spectroscopy (EIS) for a rapid and specific detection of Listeria cells. The endolysin was amine-coupled to SPEs using EDC/NHS chemistry. The CBD-based electrode was used to capture and detect the Listeria innocua serovar 6b from pure culture and 2% artificially contaminated milk. In our study, the endolysin functionalized SPEs have been characterized using X-ray photoelectron spectroscopy (XPS). The integration of endolysin-based recognition for specific bacteria and EIS can be used for direct and rapid detection of Listeria cells with high specificity against non-Listeria cells with a limit of detection of 1.1 X 10(4) and 10(5) CFU mL(-1) in pure culture and 2% milk, respectively.

引用

页码：5749 / 5756

页数：8

共 40 条

[1] Detection of Salmonella cells within 24 to 26 hours in poultry samples with the polymerase chain reaction BAX system [J].